CHO Cell-Free Protein Expression Service

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We are proud to offer our advanced CHO Cell-Free Protein Expression Service, a cutting-edge solution for the rapid and efficient production of high-quality proteins. This innovative technology leverages the advantages of Chinese Hamster Ovary (CHO) cell extracts to synthesize proteins in vitro, bypassing the limitations of traditional cell-based systems. Our CHO cell-free system is ideal for producing complex and difficult-to-express proteins, making it a valuable tool for pharmaceutical research, therapeutic development, and biotechnology applications.

Schematic overview of continuous exchange cell-free (CECF) protein synthesis based on CHO cell lysates.(L Thoring, et al.,2017)

Overview Service Process Examples and Solutions Frequently Asked Questions

Overview

we provide advanced CHO (Chinese Hamster Ovary) cell-free protein expression services, leveraging the superior capabilities of CHO cell extracts for the efficient and high-quality production of complex proteins. Our CHO cell-free system is designed to overcome the limitations of traditional cell-based expression, offering a versatile and rapid solution for producing proteins with authentic post-translational modifications.

Key Features:

High-Fidelity Protein Production: Our CHO cell-free system ensures the production of proteins with native folding and post-translational modifications, including glycosylation, phosphorylation, and more, which are essential for the proper function of many eukaryotic proteins.
Rapid Turnaround: Bypassing the need for cell culture, our cell-free system can produce proteins in a matter of hours, significantly accelerating your research timelines compared to conventional methods.
Scalability: From small-scale pilot expressions to large-scale production, our CHO cell-free system is easily scalable to meet the demands of your project, ensuring flexibility and efficiency at every stage.
Versatility: Suitable for expressing a wide range of proteins, including therapeutic proteins, enzymes, antibodies, and membrane proteins, making it an ideal choice for diverse research applications.
High Yield: Optimized conditions and advanced technology enable high-yield protein production, providing ample material for downstream applications such as structural analysis, functional assays, and therapeutic development.
Customization: Our service includes tailored optimization of expression conditions for each target protein, ensuring maximum yield and functionality.

Service Process

Project Consultation: Collaborating with researchers to define specific protein expression goals, including target proteins, desired modifications, and intended applications.
Gene Synthesis and Cloning: Synthesizing and cloning the gene of interest into suitable vectors optimized for CHO cell-free expression.
Expression Optimization: Optimizing cell-free reaction conditions to achieve high yield and functionality of proteins.
Protein Purification: Purifying produced proteins using advanced chromatography techniques to ensure high purity.
Quality Assurance: Performing stringent quality control assays to validate protein purity and functionality.
Delivery and Support: Delivering purified proteins with comprehensive documentation and providing ongoing technical support.

For more information on our CHO cell-free protein expression service, please fill out our online inquiry form. One of our specialists will get back to you promptly to discuss your specific needs and how we can assist you in achieving your goals.

Examples and Solutions

Case Examples	Solutions We Offer
Drug Discovery: Rapid production of target proteins for screening potential drug candidates.	Utilizing our CHO cell-free system to quickly produce high-quality proteins, facilitating faster drug discovery processes.
Structural Biology: Production of proteins for structural analysis.	Providing scalable production of proteins suitable for crystallization and NMR studies.
Functional Assays: Need for functional proteins for various assays.	Delivering active proteins that retain functionality, suitable for a variety of biochemical and biophysical assays.
High-Throughput Screening: Requirement of proteins for high-throughput screening assays.	Offering scalable and efficient production to meet high-demand screening needs.
Protein-Protein Interaction Studies: Investigating interactions between proteins and other biomolecules.	Producing high-quality proteins for detailed interaction studies and binding assays.
Enzyme Activity Studies: Analyzing the activity of enzymes.	Ensuring functional integrity of proteins for accurate enzymatic assays.
Vaccine Development: Production of antigens for vaccine targets.	Providing high-purity proteins for use in vaccine research and development.

Frequently Asked Questions

Q: What is CHO cell-free protein expression?

A: CHO cell-free protein expression involves synthesizing proteins in vitro using extracts from CHO cells, eliminating the need for living cells and enabling rapid and efficient production.

Q: What are the benefits of using a CHO cell-free system for protein expression?

A: CHO cell-free systems offer rapid production, flexibility, and are particularly suitable for complex proteins. They also eliminate the complexities of cell culture, making them ideal for high-throughput applications.

Q: Can you produce modified proteins using the CHO cell-free system?

A: Yes, we can produce proteins with various modifications, including tagged versions, fusion proteins, and other customized formats tailored to your specific needs.

Q: How do you ensure the quality of proteins produced?

A: We employ stringent purification and characterization techniques to ensure high purity and functionality, including quality control assays to validate protein integrity.

Q: What documentation do you provide with the delivered proteins?

A: We provide detailed reports on expression conditions, purification methods, and quality control assays, ensuring transparency and reproducibility.

Q: What kind of support do you offer post-delivery?

A: We offer ongoing technical support and troubleshooting assistance to ensure the successful utilization of the delivered proteins in your applications.

Please note that all services are for research use only. Not intended for any clinical use.

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