CLDN1 Knockout cell line （Huh-7）

The CLDN1 Knockout cell line (Huh-7) is primarily utilized in cancer research particularly in studies investigating liver cancer mechanisms and drug responses Researchers can employ this cell line to elucidate the role of claudin-1 in cellular processes such as proliferation migration and invasion A specific use case includes the assessment of targeted therapies that modulate tight junction integrity aiding in understanding therapeutic resistance in hepatocellular carcinoma (HCC)

In typical workflows researchers can implement the CLDN1 Knockout cell line in conjunction with gene editing techniques such as CRISPR-Cas9 to assess the functional impact of claudin-1 loss The practicality of using this cell line lies in its ability to produce reproducible results facilitating high-throughput screening of compounds and enhancing the predictability of in vivo responses This product excels particularly in co-culture experiments and 3D tumor spheroid assays providing insights into tumor microenvironment interactions and potential biomarkers for therapy efficacy ultimately bridging the gap between laboratory findings and clinical applications