CRISPR-Based Platforms

CD Biosynsis provides a comprehensive CRISPR-based toolbox and fully integrated platforms to power synthetic biology research. We can provide our customers with tailored solutions that meet specific research objectives and diverse applications to help them solve any problems encountered in their projects and bring breakthrough ideas to fruition.

Introduction to CRISPR-Based Technologies

CRISPR (clustered regularly interspaced short palindromic repeat) is a segment of nucleic acid containing short, repetitive base sequences derived from DNA fragments of bacteriophages that had previously infected prokaryotic cells. These sequences, found in prokaryotic genomes, play an important role in prokaryotic defense. The prokaryotic immune system consisting of CRISPR and Cas (CRISPR-associated) proteins now form the basis of genome editing technology. CRISPR-mediated genome editing is a rapidly expanding field. In addition to its widespread use in basic biology research such as gene knockdown or knock-in, gene repression or activation, genomic DNA tagging, and gene screening, the CRISPR-Cas system is also a powerful tool for the development of biological products and therapeutics.

The core concept of synthetic biology is to construct artificial decision-making circuits by building and integrating standardized components and modules. The early stage of synthetic biology mainly focused on microbial engineering, while the recent work has extended to engineering mammalian systems. As a result, tools that can more efficiently and precisely control gene expression are needed. However, the lack of sequence-specific, programmable, predictable, effective, and safe gene editing tools is an ultimate challenge in the construction of genetic circuits. Complex genetic circuits based on CRISPR allow for more precise regulation of gene expression in terms of levels and timing than traditional methods. Gene expression control based on the prokaryotic adaptive immunity system CRISPR has emerged as a powerful platform for the construction of synthetic genetic circuits.

Figure 1. Design and characterization of synthetic circuits. (Nielsen A A K & Voigt C A., 2014)

Applications

Our Platforms

CD Biosynsis offers integrated CRISPR-based platforms to help customers overcome the challenges of synthetic biology projects and accelerate their journey to success.

CD Biosynsis is committed to the establishment of advanced technology platforms and is adept at tailoring services and solutions to the customer's needs and circumstances. We are confident in the validity and reproducibility of the results we deliver because all of our services and products are based on reliable technologies and optimized protocols to meet high standards.

To start your synthetic biology projects, please feel free to contact us for more details. Our experienced scientists are available for on-demand customer assistance to help you with any concerns you may have.

References

1. Nielsen A A K & Voigt C A. Multi‐input CRISPR/C as genetic circuits that interface host regulatory networks. Molecular systems biology, 2014, 10(11): 763.
2. Xu X & Qi L S. A CRISPR–dCas toolbox for genetic engineering and synthetic biology. Journal of molecular biology, 2019, 431(1): 34-47.

Please note that all services are for research use only. Not intended for any clinical use.