Custom Antisense Oligonucleotides

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Custom Antisense Oligonucleotides are synthetic DNA or RNA molecules that are designed to specifically target and bind to complementary RNA sequences. By selectively binding to these RNA sequences, Custom Antisense Oligonucleotides can modulate gene expression and protein production, making them valuable tools in various research and therapeutic applications.

Antisense mechanism of action(ME Visser, et al.,2012) Antisense mechanism of action (ME Visser, et al.,2012)

Highlights

At our company, we offer a range of services related to Custom Antisense Oligonucleotides. Here are some key highlights of what we provide:

Tailored Design: We understand that every project is unique, and that's why we offer customized design of Antisense Oligonucleotides. Our team of experts will work closely with you to understand your specific needs and design oligonucleotides that are optimized for your target RNA.
High-Quality Synthesis: We take pride in using state-of-the-art techniques for the synthesis of our Antisense Oligonucleotides. Our advanced synthesis methods ensure that you receive oligonucleotides of the highest quality, with optimal performance and reliability.
Expert Support: Our team consists of experienced scientists who are well-versed in the field of Antisense Oligonucleotide design and synthesis. We provide expert guidance and support throughout the entire process, from initial consultation to the delivery of the final product.
Fast Turnaround Time: We understand the importance of timely delivery, and we strive to provide quick turnaround times for our customers. While the exact delivery time depends on the complexity and quantity of your order, we will provide you with an estimated timeline during the consultation phase.

Services Offered Service Process Case Studies FAQs

Services Offered

Here is a table outlining different types of Antisense Oligonucleotides (ASOs), their respective applications, and brief descriptions:

Type of ASO	Applications	Application Description	Modifications Available	Modification Descriptions
Gapmer ASOs	- Gene therapy - Cancer treatment	- Targets specific genes for mRNA degradation via RNase H activity. - Effective in long-term gene silencing.	- Phosphorothioate (PS) backbone - 2'-O-Methyl (2'-OMe) Modification - Gapmer Design	- Enhances nuclease resistance and stability - Improves pharmacokinetics and reduces immunogenicity - Combines DNA and modified RNA nucleotides
Exon Skipping ASOs	- Treatment of genetic disorders like Duchenne Muscular Dystrophy (DMD)	- Modulates splicing to skip specific exons during mRNA processing. - Restores the reading frame of mutated genes.	- Phosphorothioate (PS) backbone - 2'-O-Methyl (2'-OMe) Modification	- Enhances nuclease resistance and stability - Improves pharmacokinetics and reduces immunogenicity
siRNA-like ASOs	- Gene silencing - Antiviral therapy	- Mimics siRNA function, inducing mRNA degradation or translational repression through RISC.	- Phosphorothioate (PS) backbone - 2'-O-Methyl (2'-OMe) Modification	- Enhances nuclease resistance and stability - Improves pharmacokinetics and reduces immunogenicity
Antagomirs (miRNA Inhibitors)	- miRNA inhibition for cancer and viral diseases	- Specifically designed to inhibit microRNA (miRNA) function, regulating gene expression by preventing miRNA binding to mRNA.	- 2'-O-Methyl (2'-OMe) Modification - Locked Nucleic Acid (LNA) Modification	- Enhances stability and reduces immunogenicity - Increases binding affinity and stability
Splice Switching ASOs	- Correction of splicing errors - Treatment of diseases related to alternative splicing	- Targets pre-mRNA to influence splice site selection and correct aberrant splicing. - Used in conditions with alternative splicing.	- Phosphorothioate (PS) backbone - 2'-O-Methyl (2'-OMe) Modification - Locked Nucleic Acid (LNA) Modification - Morpholino Modification	- Enhances nuclease resistance and stability - Improves pharmacokinetics and reduces immunogenicity - Increases binding affinity and stability - Enhances stability, resistance to nucleases, and reduces non-specific interactions

Our company specializes in the production of Custom Antisense Oligonucleotides. We offer tailored design solutions, high-quality synthesis, versatile applications, and timely delivery. With our expertise and efficient processes, we can provide you with the custom antisense oligonucleotides you need for your research or therapeutic applications.

Service Process

Our service process is designed to ensure a seamless experience for our clients. Here's a step-by-step overview of how we work:

Consultation: We start by engaging in a thorough consultation with you to understand your specific requirements and goals. This initial discussion allows us to gather all the necessary information to design the most effective Antisense Oligonucleotides for your project.
Design and Optimization: Based on the information gathered during the consultation, our team of experts will design and optimize the Antisense Oligonucleotide sequences. We use cutting-edge software and algorithms to ensure the highest level of specificity and efficiency in targeting your desired RNA sequences.
Synthesis: Once the design is finalized, we move on to the synthesis phase. Our advanced synthesis techniques allow us to efficiently produce custom Antisense Oligonucleotides with high yields and purity. Throughout the synthesis process, we adhere to strict quality control measures to ensure the accuracy and integrity of the final product.
Quality Control: Quality control is a crucial step in our service process. All synthesized oligonucleotides undergo rigorous quality control measures, including analytical testing, to ensure their accuracy, purity, and functionality. We want to ensure that you receive oligonucleotides of the highest quality for your research or therapeutic applications.
Delivery: Once the quality control checks are completed, we will deliver the final product to you. Along with the custom Antisense Oligonucleotides, we provide detailed documentation that includes all the relevant information about the product, such as sequence data, purity analysis, and storage instructions.

Case Studies

We evaluated antisense oligonucleotides (ASOs) targeting Angptl3 messenger RNA (mRNA) for effects on plasma lipid levels, triglyceride clearance, liver triglyceride content, insulin sensitivity, and atherosclerosis in mice. Subsequently, 44 human participants (with triglyceride levels of either 90 to 150 mg per deciliter [1.0 to 1.7 mmol per liter] or >150 mg per deciliter, depending on the dose group) were randomly assigned to receive subcutaneous injections of placebo or an antisense oligonucleotide targeting ANGPTL3 mRNA in a single dose (20, 40, or 80 mg) or multiple doses (10, 20, 40, or 60 mg per week for 6 weeks). The main end points were safety, side-effect profile, pharmacokinetic and pharmacodynamic measures, and changes in levels of lipids and lipoproteins.

(MJ Graham, et al.,2017)

FAQs

Here are some frequently asked questions about our Custom Antisense Oligonucleotide services:

Q: How long does it take to receive the custom Antisense Oligonucleotides?

A: The turnaround time depends on the complexity and quantity of the order. We understand the importance of timely delivery and strive to provide fast turnaround times. During the consultation phase, we will provide you with an estimated timeline based on your specific project requirements.

Q: Can you provide support in the design of Antisense Oligonucleotides for specific applications?

A: Absolutely! Our team of experts has extensive experience in designing Antisense Oligonucleotides for various applications. Whether you are targeting a specific gene or need assistance in designing oligonucleotides for a particular experimental technique, we will work closely with you to ensure that the design meets your specific needs.

Q: What is the minimum order quantity for custom Antisense Oligonucleotides?

A: We do not have a strict minimum order quantity. We understand that different projects have different requirements, and we are flexible to accommodate orders of various sizes. However, please note that smaller orders may have different pricing considerations. For more details about pricing and order quantities, please contact us.

Q: Are your Antisense Oligonucleotides compatible with different experimental techniques?

A: Yes, our Antisense Oligonucleotides are compatible with a wide range of experimental techniques. We understand the diverse needs of researchers and offer oligonucleotides that can be used in various applications, including PCR, qPCR, RNA interference, and gene expression analysis. If you have any specific questions about compatibility with a particular technique, our team will be happy to provide guidance and best practices.

Q: Can you provide technical support for the use of Antisense Oligonucleotides in experiments?

A: Yes, we offer technical support to our clients. Our team of experts is available to assist you with any questions or challenges you may encounter during the experimental use of Antisense Oligonucleotides. We can provide guidance on experimental design, protocol optimization, and troubleshooting.

Q: What are the storage requirements for custom Antisense Oligonucleotides?

A: To maintain the stability and integrity of the Antisense Oligonucleotides, it is recommended to store them at -20°C or below. When received, the oligonucleotides will be provided with detailed storage instructions to ensure their long-term stability. It is important to handle and store the oligonucleotides according to the provided guidelines.

Q: Can you assist with the delivery of Antisense Oligonucleotides to international locations?

A: Yes, we have experience in shipping Antisense Oligonucleotides internationally. We will work closely with you to ensure that the necessary customs documentation and shipping regulations are followed. Please note that shipping times and costs may vary depending on the destination. For more information and assistance with international shipments, please contact our team.

Q: Do you offer customization options for Antisense Oligonucleotide modifications?

A: Yes, we provide customization options for Antisense Oligonucleotide modifications. We offer a range of modifications, such as phosphorothioate backbone, 2'-O-methyl RNA, locked nucleic acids (LNAs), and more. These modifications can enhance stability, nuclease resistance, and target specificity. Our team can discuss the available options and help you choose the most suitable modifications for your specific needs.

We are committed to providing high-quality Custom Antisense Oligonucleotides and exceptional service to our clients. If you have any further questions or would like to discuss your project in more detail, please don't hesitate to contact us.

Please note that all services are for research use only. Not intended for any clinical use.

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