Custom CHO Cell Protein Expression Service

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Our Custom CHO Cell Protein Expression Service offers specialized solutions for the production of recombinant proteins in Chinese Hamster Ovary (CHO) cells, a highly efficient and versatile host for industrial-scale protein production. Utilizing advanced expression technologies and optimization strategies, we provide comprehensive support from project design to final protein purification, ensuring your protein expression goals are achieved with high accuracy and efficiency.

CHO cell protein expression(B Tihanyi, et al.,2020)

Overview Service Process Examples and Solutions Frequently Asked Questions

Overview

CHO cells are a prominent host for recombinant protein expression in biotechnology due to their ability to produce large quantities of proteins with proper post-translational modifications. These cells are known for their robustness, scalability, and capacity to perform human-like glycosylation, making them ideal for producing therapeutic proteins and other complex biopharmaceuticals. CHO cells are widely used in various applications, including pharmaceuticals, biotechnology, and research.

Advantages

High Protein Yield: CHO cells can produce large amounts of recombinant proteins, suitable for commercial production.
Human-like Glycosylation: CHO cells can perform post-translational modifications, including glycosylation, which is crucial for the functionality of many therapeutic proteins.
Scalability: CHO cell cultures can be easily scaled up, making them suitable for large-scale industrial production.
Regulatory Acceptance: CHO cells are widely accepted by regulatory agencies for the production of biopharmaceuticals, ensuring compliance with industry standards.
Versatility: Applicable for producing a wide range of proteins, including monoclonal antibodies, enzymes, and other therapeutic proteins.
Robustness: CHO cells are known for their stability and robustness in various culture conditions.

By leveraging these advantages, CHO cell expression systems provide an efficient and cost-effective platform for producing high-quality recombinant proteins, meeting the needs of various industrial and research applications.

Service Process

The process of custom CHO cell expression involves several critical and interrelated steps:

Project Consultation: Collaborating with researchers to define specific protein expression goals, including target proteins, desired modifications, and intended applications.
Gene Synthesis and Cloning: Synthesizing and cloning the gene of interest into suitable expression vectors optimized for CHO cells.
Transfection: Introducing the expression vectors into CHO cells using techniques such as electroporation or lipofection.
Expression Optimization: Optimizing expression conditions, including selection of high-producing clones, induction methods, and media composition, to maximize protein yield and functionality.
Protein Purification: Purifying the expressed proteins using techniques such as affinity chromatography, ion exchange chromatography, and size-exclusion chromatography.
Quality Control: Performing quality control assays to ensure the purity, concentration, and activity of the expressed proteins. This includes SDS-PAGE, Western blotting, and activity assays.
Characterization: Characterizing the expressed proteins for structural and functional properties. This may include mass spectrometry, NMR, and X-ray crystallography.
Reporting and Consultation: Providing a detailed report of the findings and offering further consultation to interpret the results and plan subsequent research steps.

For more information about our Custom CHO Cell Expression Service or to discuss your specific needs, please contact us. Our team of experts is available to provide guidance and support for your research and biotechnological projects, ensuring you achieve your scientific and industrial goals.

Examples and Solutions

The following table provides an overview of various case studies in CHO cell protein expression and the solutions we offer to support your research and biotechnological endeavors:

Case Study Description	Solutions We Offer
Therapeutic Protein Production	Producing recombinant therapeutic proteins for pharmaceutical applications. Gene synthesis, expression optimization, protein purification, and quality control.
Monoclonal Antibody Production	Generating monoclonal antibodies for therapeutic and diagnostic uses. Expression vector design, host cell optimization, protein purification, and activity assays.
Enzyme Production for Industrial Use	Producing enzymes for applications in biofuels, food processing, and waste management. Gene cloning, expression optimization, protein purification, and quality assurance.
Diagnostic Protein Production	Producing proteins for use in diagnostic assays and kits. Gene synthesis, expression optimization, protein purification, and quality control.
Structural Biology Studies	Producing proteins for structural studies such as X-ray crystallography and NMR. Gene synthesis, expression optimization, protein purification, and structural characterization.
Biopharmaceutical Development	Developing complex biopharmaceuticals for various therapeutic applications. Gene design, expression optimization, protein purification, and functional assays.

Frequently Asked Questions

Q: What is CHO cell protein expression?

A: CHO cell protein expression involves using Chinese Hamster Ovary (CHO) cells to produce large quantities of recombinant proteins. These cells are known for their ability to perform human-like glycosylation, making them ideal for producing therapeutic proteins.

Q: How is CHO cell protein expression performed?

A: CHO cell protein expression is performed through a series of steps including project consultation, gene synthesis and cloning, transfection, expression optimization, protein purification, quality control, characterization, and reporting. Each step ensures precise and efficient protein production.

Q: What are the applications of CHO cell protein expression?

A: Applications include pharmaceutical production, monoclonal antibody generation, industrial enzyme production, diagnostic assays, structural biology studies, and biopharmaceutical development. Expressed proteins are used for therapeutic applications, industrial processes, and research purposes.

Q: What are the key steps in the CHO cell protein expression process?

A: Key steps include project consultation, gene synthesis and cloning, transfection, expression optimization, protein purification, quality control, characterization, and reporting. These steps ensure comprehensive and accurate protein production.

Q: Why is CHO cell protein expression important?

A: CHO cell protein expression is important for advancing research, developing new therapies, optimizing industrial processes, and creating innovative biotechnological solutions. Recombinant proteins produced in CHO cells are essential tools in various scientific and industrial applications.

Please note that all services are for research use only. Not intended for any clinical use.

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