Custom DNA oligos are short, single-stranded DNA sequences that are synthesized to order. They play a crucial role in various fields of molecular biology research, genetic testing, and biotechnology applications. These oligos are designed with specific sequences to meet the unique requirements of researchers and scientists.
Custom DNA oligos are versatile tools used in various molecular biology applications, and their quality, sequence accuracy, and purity are critical for the success of downstream experiments. Below is a table outlining the application areas and requirements for custom DNA oligos:
Application Area | Description | Requirements |
---|---|---|
PCR Amplification | Primer sequences for polymerase chain reaction (PCR). | Knowledge of target sequence, primer design software, DNA synthesizer, suitable reagents (nucleotides, phosphoramidites). |
DNA Sequencing | Sequencing primers for Sanger or next-generation sequencing. | Design of sequencing primers, DNA synthesizer, high-quality reagents, purification methods, compatibility with sequencing platform. |
Gene Synthesis | Construction of synthetic genes for various purposes. | Accurate sequence design, DNA synthesizer, high-quality reagents, purification methods, sequence verification. |
Site-Directed Mutagenesis | Introduction of specific mutations into DNA sequences. | Design of mutagenic primers, DNA synthesizer, reagents for PCR, sequence verification methods. |
Probe Hybridization | Production of probes for molecular detection applications, such as fluorescence in situ hybridization (FISH). | Design of probe sequence, labeled nucleotides or post-synthesis labeling, purification methods, quality control. |
Amplicon Sequencing | Primers for targeted sequencing of specific genomic regions. | Design of amplicon-specific primers, DNA synthesizer, high-quality reagents, purification methods, compatibility with sequencing platform. |
Real-Time PCR (qPCR) | Primers and probes for quantitative PCR applications. | Design of qPCR primers and probes, DNA synthesizer, high-quality reagents, purification methods, compatibility with qPCR instrumentation. |
Functional Genomics | Synthesis of custom oligos for various functional genomics studies. | Design of oligo sequences, DNA synthesizer, purification methods, validation assays. |
We are committed to providing you with high-quality custom DNA oligos that meet your specific research needs. Our experienced team, advanced technology, fast turnaround times, and competitive pricing make us the ideal partner for all your custom DNA oligo requirements. Contact us today to discuss your project and let us help you advance your research!
Here are some commonly asked questions about our custom DNA oligos:
A: The turnaround time for custom DNA oligos can vary depending on the specific requirements and order volume. However, we prioritize timely delivery and strive to provide an estimated timeframe for completion. Please contact our team for more information regarding your specific project.
A: Absolutely! Our team of experts is available to provide design consultation and support throughout the entire process. We will work closely with you to understand your research goals and help you design the DNA oligos that meet your specific requirements.
A: To maintain the stability and integrity of the DNA oligos for long-term use, we recommend storing them at -20°C. This temperature ensures that the oligos remain in optimal condition and are ready for use whenever you need them.
A: Custom DNA oligos have a wide range of applications in molecular biology research and biotechnology. They can be used for techniques such as PCR (Polymerase Chain Reaction), DNA sequencing, gene expression analysis, molecular cloning, and much more. The versatility of custom DNA oligos makes them essential tools for various research applications.
A: Yes, we offer options for modified bases and labels for your custom DNA oligos. We understand that specific modifications or labels may be required for your research project.
A: We offer various purification options for custom DNA oligos, including standard desalting, HPLC purification, and PAGE purification. The choice of purification method depends on the specific requirements of your research project. Our experts can guide you in selecting the most suitable purification method for your needs.
A: Absolutely! Our team of experts is available to provide technical support and troubleshooting assistance for experiments involving DNA oligos. We understand that research experiments can sometimes encounter challenges, and we are here to help you overcome them.
A: Yes, we offer bulk discounts for large orders of custom DNA oligos. Please contact our team to discuss your order volume and specific requirements, and we will provide you with a competitive pricing quote.
A: We have stringent quality control measures in place to ensure the accuracy and purity of our custom DNA oligos. Our quality control includes analytical testing to verify the sequence and length of the oligos, as well as purity assessment to ensure minimal levels of impurities.
A: Yes, we can provide documentation for the quality control tests performed on the DNA oligos upon request. Our goal is to ensure transparency and provide you with the necessary information to validate the quality of our products.
A: When stored properly at -20°C, our custom DNA oligos have a shelf life of up to 24 months. We recommend following proper storage guidelines to maintain the stability and integrity of the oligos throughout their shelf life.
A: Yes, our team can assist you with the design of primers for PCR experiments. We have extensive experience in primer design and can help you optimize the primer sequences for your specific PCR applications.
A: Yes, we offer DNA oligos with modified backbones for specific applications. Modified backbones can enhance stability, nuclease resistance, or other properties based on your research needs. Please discuss your requirements with our team, and we will assist you accordingly.
A: we prioritize the confidentiality and privacy of our customers' sequences. We have strict data protection measures in place to ensure that your sequences and research information remain confidential. We adhere to industry-standard security practices and protocols to safeguard your data.
A: Yes, we can provide a certificate of analysis (CoA) for the custom DNA oligos upon request. The CoA includes detailed information about the synthesis, purification, and quality control results of the oligos.
Please note that all services are for research use only. Not intended for any clinical use.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.