Custom Protein Production Services

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Custom protein production services offer tailored solutions for the expression and purification of recombinant proteins to meet specific research and industrial needs. Our services provide comprehensive support from gene synthesis to final protein characterization, ensuring that your proteins are produced with high quality, purity, and functionality. Whether you need proteins for therapeutic development, structural studies, or industrial applications, our custom protein production services are designed to meet your unique requirements.

Overview Service Process Examples and Solutions Frequently Asked Questions

Overview

Custom protein production involves the design, expression, purification, and characterization of proteins based on client specifications. Our services utilize a variety of expression systems, including bacterial, yeast, insect, and mammalian cells, to produce proteins with desired post-translational modifications and functionalities. We employ advanced techniques and state-of-the-art facilities to deliver high-quality proteins efficiently and reliably.

Cultivation strategies choice based on the promoter utilized for recombinant gene expression. (AM Vieira Gomes, et al.,2018)

Methods for Custom Protein Production

Method	Description	Applicable Scenarios
Bacterial Expression Systems	Utilizes bacteria, typically E. coli, to express recombinant proteins due to their rapid growth and high yield capabilities.	Suitable for producing large quantities of protein quickly and cost-effectively, ideal for initial screening and large-scale production.
Yeast Expression Systems	Employs yeast cells, such as Saccharomyces cerevisiae or Pichia pastoris, for protein expression, offering eukaryotic post-translational modifications.	Useful for producing proteins with complex post-translational modifications, such as glycosylation, suitable for both research and industrial applications.
Insect Cell Expression Systems	Uses baculovirus-infected insect cells (e.g., Sf9, Sf21) to produce recombinant proteins with proper eukaryotic folding and modifications.	Ideal for producing proteins that require complex post-translational modifications and proper folding, often used in structural biology and vaccine production.
Mammalian Cell Expression Systems	Involves the use of mammalian cell lines (e.g., HEK293, CHO) to produce proteins with native folding and human-like post-translational modifications.	Essential for producing therapeutic proteins, antibodies, and proteins requiring human-like modifications, widely used in pharmaceutical development.
Cell-Free Expression Systems	Employs cell-free extracts to synthesize proteins in vitro, offering rapid and scalable protein production without the need for living cells.	Suitable for rapid protein production, high-throughput screening, and situations where toxic or unstable proteins are produced.
Transient Expression Systems	Involves the temporary expression of proteins in cells, allowing for rapid production without the need for stable cell line development.	Useful for quick production of proteins for preliminary studies, functional assays, and small-scale experiments.
Stable Cell Line Development	Generates cell lines that stably express the protein of interest, ensuring consistent and long-term protein production.	Ideal for large-scale and long-term production of therapeutic proteins, monoclonal antibodies, and other biopharmaceuticals.
Fusion Protein Expression	Utilizes fusion tags (e.g., His-tag, GST-tag) to enhance the solubility and facilitate the purification of recombinant proteins.	Suitable for simplifying protein purification processes, improving yield, and enabling easy detection and quantification.
Multigene Expression Systems	Allows for the simultaneous expression of multiple proteins, enabling the production of protein complexes and pathways in a single host.	Ideal for studying protein-protein interactions, metabolic engineering, and producing multi-subunit complexes.

Each method in custom protein production is chosen based on the specific requirements of the target protein, such as the need for post-translational modifications, yield, speed, and the intended application. The flexibility in choosing the appropriate system ensures the efficient and effective production of high-quality recombinant proteins.

Service Process

The process of custom protein production involves several critical and interrelated steps:

Project Consultation: Collaborating with clients to define specific protein production requirements, including target protein function, expression system, and purification needs.
Gene Synthesis and Cloning: Designing and synthesizing the gene encoding the target protein, followed by cloning into an appropriate expression vector. Verification through sequencing ensures accuracy.
Expression Vector Construction: Constructing expression vectors tailored to the selected host system (bacterial, yeast, insect, or mammalian cells) to achieve optimal protein expression.
Protein Expression: Introducing the expression vector into the chosen host system and optimizing conditions to maximize protein yield. Parameters such as temperature, inducer concentration, and growth media are adjusted.
Protein Purification: Isolating the expressed protein from host cells using techniques such as affinity chromatography, size-exclusion chromatography, or ion exchange chromatography. Purification strategies are tailored to the specific properties of the protein.
Characterization and Validation: Assessing the purity, stability, and functionality of the expressed protein using methods such as SDS-PAGE, Western blotting, mass spectrometry, and activity assays.
Optimization and Scale-Up: Refining the production process based on initial results and scaling up production to meet the required quantities for research or commercial use.

For more information about our Custom Protein Production Services or to discuss your specific needs, please contact us. Our team of experts is available to provide guidance and support for your research and biotechnological projects, ensuring you achieve your scientific and industrial goals.

Examples and Solutions

The following table provides an overview of various case studies in custom protein production and the solutions we offer to support your research and biotechnological endeavors:

Case Study	Description	Solutions We Offer
Monoclonal Antibody Production	Producing monoclonal antibodies for therapeutic and diagnostic applications.	Gene synthesis, vector construction, protein expression, and purification.
Vaccine Antigen Production	Developing viral antigens for vaccine research and development.	Custom expression systems, purification, and validation.
Structural Protein Expression	Expressing proteins for structural studies such as X-ray crystallography and cryo-EM.	High-yield expression, purification, and structural characterization.
Industrial Enzyme Production	Producing enzymes with enhanced stability and catalytic efficiency for industrial processes.	Gene synthesis, expression optimization, and activity assays.
Diagnostic Protein Development	Manufacturing proteins for use in ELISA and other diagnostic assays.	Custom labeling, assay development, and validation.
Agricultural Biotechnology Proteins	Developing proteins to enhance pest resistance or stress tolerance in plants.	Gene design, plant transformation, and functional validation.

Frequently Asked Questions

Q: What is custom protein production?

A: Custom protein production involves the design, expression, purification, and characterization of proteins based on client specifications. This service provides tailored solutions to produce high-quality proteins for various applications.

Q: How is custom protein production performed?

A: Custom protein production is performed through a series of steps including project consultation, gene synthesis and cloning, expression vector construction, protein expression, protein purification, characterization and validation, and optimization and scale-up. Each step ensures the accurate and efficient production of functional proteins.

Q: What are the applications of custom protein production?

A: Applications include therapeutic protein development, structural biology, enzyme engineering, biotechnology, synthetic biology, and agricultural biotechnology. Custom-produced proteins are essential for research, therapeutic, and industrial purposes.

Q: What are the key steps in the custom protein production process?

A: Key steps include project consultation, gene synthesis and cloning, expression vector construction, protein expression, protein purification, characterization and validation, and optimization and scale-up. These steps ensure the successful production of high-quality recombinant proteins.

Q: Why is custom protein production important?

A: Custom protein production is important for advancing research, developing new therapies, improving industrial processes, and creating innovative biotechnological tools. It provides tailored solutions for specific protein production challenges.

Please note that all services are for research use only. Not intended for any clinical use.

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