Custom Protein Purification from E. coli Service

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Overview Core Service Offerings Application Areas Project Workflow Case Studies Frequently Asked Questions (FAQs) Contact Us

Overview

Our company is a professional CRO service outsourcing company committed to providing customers with high-quality customized protein purification services. Our E. The coli protein purification service provides His-tag or GST-tag options from gene synthesis to labeled protein purification, with purities of 85%, 90% or 95%, and quantities ranging from milligrams to grams.

E. coli is currently the most commonly used and cost-effective recombinant protein expression system, and its growth rate far exceeds that of insects, mammalian cells or yeast, making it possible to quickly test a large number of different conditions. Our services not only include protein expression and purification, but also cover subsequent modification and quality control to ensure that each batch of protein has high purity, biological activity and batch-to-batch consistency.

In addition, we also provide a variety of purification systems and post-processing services, such as desalination, sub-packaging, endotoxin removal, aseptic processing and freeze-drying, to meet the different needs of customers. Our project management team is experienced and able to efficiently coordinate the entire process to ensure smooth progress and high-quality delivery of the project.

Whether you need small-scale high-throughput screening or large-scale production, our E. coli protein purification services can provide you with comprehensive support and solutions. Choose us and you will receive professional, efficient and reliable customized protein purification services.

Comparison of aYnity tags to purify tagged DHFR proteins expressed in E. coli(JJ Lichty, et al.,2005)

Core Service Offerings

Custom Protein Purification from E. coli services are a one-stop solution covering the entire process from gene synthesis to protein purification. Our one-stop service solutions include the following:

From gene to protein: We provide full-process services from gene synthesis to protein expression and purification to ensure that customers can obtain high-quality recombinant proteins.
High-throughput and large-scale production: Our production capacity ranges from 2 liters to 1000 liters and can meet customer needs of different sizes.
Multiple purification systems: We provide multiple protein purification systems to ensure that customers can obtain high-purity protein products.
Post-processing services: After protein purification, we also provide post-processing services such as desalination, sub-packaging, endotoxin removal, sterile processing and freeze-drying to ensure product quality and safety.
Label selection and purity assurance: Customers can choose between His tag or GST tag, and we guarantee protein purity of 85%, 90% or 95%. If no protein is produced, we will not charge any fees to ensure a high success rate.
Project Management Team: We have a dedicated and experienced project management team to ensure the smooth progress and efficient completion of projects.

Through these one-stop services, we are committed to providing customers with efficient, professional and reliable Custom Protein Purification from E. coli solutions to help customers accelerate their research and development processes.

Application Areas

Our Custom Protein Purification from E. coli services have a wide range of application areas and uses, covering multiple scientific research and industrial fields. Here is a detailed summary of these application areas:

Drug research and development: E. The coli system is often used to produce recombinant proteins that can be used for functional analysis, pharmacological research, and drug safety evaluation in drug development.
For example, through E. The recombinant protein produced by the coli expression system can be used to develop antibody drugs, vaccines and nucleic acid drugs.
Structural biology: E. coli is the expression system of choice for many structural biology research. Through high-throughput protein expression and purification processes, researchers are able to obtain high-quality protein samples for X-ray crystallography or nuclear magnetic resonance (NMR) analysis.
Enzyme research: E. coli system is widely used in enzyme discovery and engineering research. Through low-cost small-scale expression and purification processes, researchers can quickly screen and optimize enzyme activity and stability.
Bioanalysis and testing: E. coli can be used to develop various biosensors and Detection Tools for early diagnosis of diseases and detection of biomarkers.
Protein engineering: E. The coli system is often used in protein engineering research. Through gene cloning and expression optimization, researchers can perform mutation analysis and functional improvement on specific proteins.
Industrial applications: E. coli expression systems are widely used in industrial production because of their simplicity, speed and cost-effectiveness. For example, through E. The recombinant protein produced by the coli expression system can be used in fields such as biocatalysis and biomanufacturing.
Basic scientific research: E. The coli system is an important tool for many basic scientific research, used to study the mechanisms of protein biosynthesis, folding and degradation.

In summary, Custom Protein Purification from E. coli services are widely used in many fields such as drug research and development, structural biology, enzymology research, biological analysis and testing, protein engineering, industrial applications, and basic scientific research.

Project Workflow

The service process for Custom Protein Purification from E. coli Service provided by our company is as follows:

Gene sequence optimization: Codon optimization is carried out according to the gene sequence provided by the customer to improve expression efficiency in Escherichia coli.
Construction of recombinant plasmid: Use the optimized gene sequence to construct the recombinant plasmid, and select the appropriate E. coli cell strain.
Recombinant protein expression: By optimizing temperature, IPTG concentration, culture time and other conditions, E. coli cells express the recombinant protein. We provide a variety of recombinant plasmids and transformed host strains to ensure high expression levels.
Soluble expression condition testing: Test soluble expression under different conditions to ensure that high-purity soluble protein is obtained.
Small-scale protein expression and purification: Carry out small-scale protein expression and purification experiments to verify the expression and purification effects.
Large-scale protein production: Carry out large-scale protein production based on the results of small-scale experiments to ensure the demand for high-throughput, large-scale protein production.
Protein purification: Adopt a variety of purification technologies (such as Ni-NTA purification technology) to ensure that protein purity meets customer requirements.
Protein renaturation: For inclusion body proteins, renaturation is carried out to restore their activity.
Endotoxin removal: Provide additional services such as endotoxin removal to meet customers 'high requirements for protein purity and safety.
Quality control: Conduct quality control on the finally purified protein to ensure that its molecular weight, purity, etc. meet expectations.

Through the above process, we can provide customers with one-stop services from gene sequencing to protein purification, ensuring efficient and high-quality recombinant protein production.

Case Studies

Customer background:

A biotechnology company needs to mass produce a recombinant protein for drug development. Their main challenge is how to obtain high-quality purified proteins in a short period of time.

Solution:

Our company provides customized purified protein services from Escherichia coli. By utilizing advanced fusion labeling technology and single column purification methods, we have successfully obtained E The target protein was extracted and purified from Escherichia coli. Our client obtained high-quality purified protein within just one week, and the purification process met international standards.

Achievements:

The customer is very satisfied with our service, not only because we provided high-quality purified protein in a short period of time, but also because of the technical support and follow-up services we provided. This has enabled them to make significant progress in the drug development process.

Frequently Asked Questions (FAQs)

In order to help customers better understand and use the Custom Protein Purification from E. coli service provided by your company, the following are some common questions and their answers:

1. What is E Coli protein expression and purification services?

A: E. coli protein expression and purification service is a technology that uses E. coli as a host cell to express and purify recombinant proteins. This method is widely used due to its low cost, fast growth rate, high expression level, and easy purification.

2. What is the service process of your company?

A: Our process starts with gene synthesis, goes through protein expression, and finally undergoes protein purification. We offer multiple labeling options (such as His tags or GST tags) and provide protein products of different purities (85%, 90%, 95%) according to customer needs. In addition, we also offer protein yields ranging from milligrams to grams.

3. How does your company ensure the purity and activity of proteins?

A: We adopt high-throughput and large-scale production technologies, ranging from 2 liters to 1000 liters. Our purification systems are diverse, including post purification services such as desalination, packaging, endotoxin removal, aseptic treatment, and freeze-drying. In addition, our project management team is experienced in ensuring that each batch of protein has high purity and biological activity.

4. What is the service price of your company?

A: We offer a variety of service packages, with specific prices depending on factors such as protein purity, production volume, and label type.

5. What are the advantages of your company compared to other CRO companies?

A: Our advantage lies in our professionalism and experience. We not only provide one-stop services from genes to proteins, but also have high-throughput and large-scale production capabilities. In addition, our project management team is experienced and capable of efficiently handling various complex project requirements.

By organizing the above common questions and answers, we hope to help customers better understand and use your company's Custom Protein Purification from E. coli service.

Contact Us

By choosing our service, you will experience the simplest, fastest, and most cost-effective protein expression system. We have over 20 purification units that support multi-step protein purification, ensuring that your project can be efficiently completed. In addition, our service package provides risk protection, and if protein is not obtained, no fees need to be paid, with a success rate of up to 95%.

If you need high-quality protein purification services, please feel free to contact us at any time. We look forward to working with you to jointly promote the success of your research project. Contact us immediately.

Please note that all services are for research use only. Not intended for any clinical use.

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