Custom sgRNA Libraries

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Custom sgRNA Libraries are specialized tools used in gene editing research. They consist of a collection of small guide RNAs (sgRNAs) that are designed to target specific genes of interest. These libraries are essential for performing targeted genetic modifications and studying gene function.

We offer a comprehensive range of services for custom sgRNA Libraries. Our team of experts will work closely with you to design and deliver high-quality sgRNA Libraries tailored to your specific research needs.

Service Process Case Studies FAQs

Service Process

Our service process is designed to ensure a seamless experience for our clients. Here is an overview of the steps involved:

Consultation: Our team will schedule a consultation with you to discuss your research goals and requirements. This step is crucial for us to understand the specific genes and modifications you are targeting.
Design: Based on the information gathered during the consultation, our team of experts will design a customized sgRNA Library. We will take into account factors such as library size, target gene selection, and delivery format to meet the unique requirements of your research project.
Synthesis and Validation: Once the design is finalized, we will proceed with the synthesis of the sgRNA Library. Our rigorous validation process ensures the quality and efficiency of the library, providing you with reliable tools for your gene editing experiments.
Delivery: The final sgRNA Library will be delivered to you in the desired format, ready for use in your experiments. We ensure secure packaging and prompt delivery to ensure the integrity of the library during transit.

Case Studies

Generation of KO cell lines utilizing the sgRNAKOLIBRY library. (a) Schematic diagram of knockout cell line generation. (b) Schematic view of the genetic locus of the human TBKBP1 gene. The red arrow indicates the location of the target site of the sgRNA. The red sequence highlights the target site whereby the orange sequence indicates the PAM. (c) OutKnocker was used to analyze deep sequencing data obtained from gene targeting experiments. Each pie chart represents the deep sequencing data from an individual cell clone. Each pie piece sums up all reads that have the same indel mutation, with its size representing the proportion of total reads analyzed. Grey pie charts show reads without indel mutations, blue colors indicate indel mutations that are in frame and red colors indicate indel mutation reads that are out of frame, whereas the different shades of blue and red represent individual indel mutations in one pie chart. Depicted is an exemplary output for the generation of TBKBP1 knockout HEK cells. (d) Shown is a histogram summarizing the results of 19 individual gene targeting projects. Grey bars show the number of clones that carry only unmodified alleles, while purple bars indicate clones with at least one mutated allele and red bars clones with all-allelic frame shift mutations.

(T Schmidt, et al.,2015)

FAQs

Here are some frequently asked questions about our custom sgRNA Libraries:

Q: What is the turnaround time for receiving the custom sgRNA Libraries?

A: The turnaround time can vary depending on the complexity and size of the library. Our team will provide you with an estimated timeline during the consultation phase. We strive to deliver your custom sgRNA Libraries as quickly as possible, without compromising on quality.

Q: Can I customize the target genes for the sgRNA Libraries?

A: Yes, we offer the flexibility to select specific target genes according to your research requirements. Our team will guide you through the gene selection process to ensure that the sgRNA Libraries are designed to meet your specific needs.

Q: Do you provide support for library screening and data analysis?

A: Yes, our team can assist you with library screening and provide guidance on data analysis to help you interpret the results effectively. We understand that analyzing the data obtained from your experiments is crucial for drawing meaningful conclusions, and we are here to support you throughout the process.

Q: Are your sgRNA Libraries suitable for various gene editing techniques?

A: Absolutely! Our sgRNA Libraries are compatible with a wide range of gene editing techniques, including CRISPR-Cas9, CRISPR-Cas12a, and more. Whether you are using established methods or exploring the latest gene editing technologies, our custom sgRNA Libraries will provide you with the necessary tools for success.

Q: What is the recommended concentration for using the sgRNA Libraries in gene editing experiments?

A: The recommended concentration for using the sgRNA Libraries may vary depending on the specific experiment and gene editing technique employed. Our team will provide you with guidelines and recommendations tailored to your research objectives during the consultation phase.

Q: Can the sgRNA Libraries be used in both in vitro and in vivo experiments?

A: Yes, our sgRNA Libraries can be used in both in vitro and in vivo experiments. Whether you are studying gene function in cell lines or conducting gene editing in live organisms, our libraries are designed to provide efficient and specific targeting of your genes of interest.

Q: What is the shelf life of the sgRNA Libraries?

A: The shelf life of the sgRNA Libraries depends on the specific storage conditions. We provide detailed storage instructions with each delivery to ensure the stability and longevity of the libraries. Typically, when stored properly at -20°C, the libraries remain stable for several months.

Q: Can I request modifications to the sgRNA Libraries after delivery?

A: Modifications to the sgRNA Libraries after delivery may not be possible due to the nature of the synthesis process. However, our team is committed to working closely with you during the design phase to ensure that the libraries meet your requirements and expectations.

Q: Do you offer technical support for using the sgRNA Libraries?

A: Yes, we provide comprehensive technical support for using our sgRNA Libraries. Our team of experts is available to answer any questions you may have regarding library usage, experimental design, troubleshooting, and data interpretation.

If you have any further questions or would like to discuss your specific project in detail, please feel free to contact us. Our team of experts is ready to assist you in achieving your gene editing research goals.

Please note that all services are for research use only. Not intended for any clinical use.

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