DNA Assembly and Cloning Services

Through DNA assembly and cloning services, CD Biosynsis aims to provide researchers and innovators with streamlined access to complex genetic constructs, which will alleviate the technological challenges associated with DNA manipulation and enable researchers to focus on their core objectives, facilitating their breakthroughs in different fields.

Introduction to DNA Assembly and Cloning

DNA assembly and cloning form the bedrock of modern synthetic biology endeavors. DNA assembly involves the construction of DNA molecules by combining smaller DNA fragments in a precisely orchestrated manner. This process enables the creation of novel genetic sequences, thereby facilitating the design and engineering of organisms with tailored functionalities. Cloning, on the other hand, involves the replication of a DNA fragment or an entire DNA molecule to produce identical copies.  These techniques are cornerstones of genetic manipulation, enabling researchers to delve into the fundamental mechanisms of life and engineer biological systems for a myriad of applications.

Fig 1. Generate plant knock-out mutants by using CRISPR-Cas9 mediated genome editing. (Zhang Y, et al., 2020)

DNA Assembly and Cloning: Unveiling the Technique

Advancements in DNA synthesis technologies have allowed researchers to access custom-designed DNA fragments, reducing the reliance on naturally occurring DNA sources. Current trends in DNA assembly and cloning underscore an increasing integration of computational tools. Computer-aided design (CAD) software enables the rapid virtual assembly of DNA fragments, streamlining the design process. The rise of automation and microfluidic platforms has further accelerated assembly procedures, minimizing human intervention and error. In the realm of cloning, CRISPR-Cas9 technology has revolutionized gene editing, enabling precise modification of DNA sequences and the creation of genetically modified organisms with unprecedented ease.

Our Technologies

• Automated Liquid Handling. Precision is ensured through automated liquid handling systems that dispense reagents accurately, minimizing errors and increasing throughput.
• Bioinformatics Integration. Bioinformatics tools expedite sequence analysis, gene design, and optimization, aiding in informed genetic component selection.
• CRISPR-Cas9 and Synthetic Biology Fusion. The fusion of CRISPR-Cas9 genome editing with synthetic biology enhances precision and versatility in genetic engineering.

Process of DNA Assembly and Cloning Services

Project Design. The process commences with project design, encompassing DNA fragment selection, vector design, and sequence optimization.

Synthesis or Isolation. Upon design approval, DNA synthesis or isolation takes place, ensuring fidelity and purity of genetic material.

Assembly Process. Advanced assembly techniques integrate genetic fragments seamlessly, leading to the emergence of chimeric DNA constructs.

Validation. Construct validation includes functional assays and analytical techniques to guarantee conformity with predefined specifications.

Competitive Advantages

• Scientific and Technological Synergy. Our platform's fusion of scientific expertise and technological adeptness enables handling projects of varying complexities.
• Modular Assembly and CRISPR-Cas9 Precision. Integration of modular assembly and CRISPR-Cas9 techniques ensures unprecedented accuracy in outcomes.
• Quality Assurance Commitmen. Our dedication to quality assurance guarantees that delivered constructs embody precision, reliability, and functionality.

CD Biosynsis specializes in providing exceptional services in the field of synthetic biology. By choosing our service platform, you have access to our expertise, advanced technologies, and personalized support to take your research to new heights. Contact us to know more details about our services.

Reference

1. Zhang Y and Showalter AM. CRISPR/Cas9 Genome Editing Technology: A Valuable Tool for Understanding Plant Cell Wall Biosynthesis and Function. Front. Plant Sci. 2020, 11:589517.

Please note that all services are for research use only. Not intended for any clinical use.