EGFP Stable Cell Line

EGFP Stable Cell Lines are genetically engineered cells that consistently express the Enhanced Green Fluorescent Protein (EGFP). EGFP is a widely used fluorescent marker derived from the green fluorescent protein (GFP), offering improved brightness and photostability. These cell lines are created by integrating the EGFP gene into the cell’s genome, ensuring stable and continuous expression of the green fluorescence. EGFP Stable Cell Lines are powerful tools in cell biology, enabling researchers to visualize and track cellular processes, protein localization, and gene expression in real time without disrupting normal cellular functions.

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Products Application Supporting Data Resources Related Products

Products

Catalog Number	Product Name	Price
EC0003	Human colon adenocarcinoma cells (RKO-EGFP)	$1066.67
EC0004	Human esophageal squamous cell carcinoma cells (KYSE-30-EGFP)	$1191.67
EC0005	Porcine small intestine epithelial cells (IPEC-J2-EGFP)	$1191.67
EC0006	Human liver cancer cells (HuH-7-EGFP)	$1191.67
EC0007	Mouse liver cancer cells (Hepa 1-6-EGFP)	$1066.67
EC0008	Human liver cancer cells (Hep G2-EGFP)	$1066.67
EC0009	Human colon cancer cells (HT-29-EGFP)	$1066.67
EC0010	Human colon cancer cells (HCT 116-EGFP)	$1066.67
EC0011	Human gastric adenocarcinoma cells (AGS-EGFP)	$1066.67
EC0012	Human renal cell adenocarcinoma cells (ACHN-EGFP)	$1191.67
EC0013	Human colon adenocarcinoma cells (SW480-EGFP)	$1191.67
EC0014	Human breast adenocarcinoma cells (SK-BR-3-EGFP)	$1191.67
EC0015	Human breast cancer cells (MDA-MB-231-EGFP)	$1191.67
EC0016	Human prostate cancer cells (LNCaP Clone FGC-EGFP)	$1191.67
EC0017	Human breast cancer cells (HCC1806-EGFP)	$1191.67

Application

EGFP Stable Cell Lines are commonly used in a variety of applications, including live-cell imaging, where they allow for the observation of dynamic cellular processes such as protein trafficking, cell division, and signal transduction. In gene expression studies, EGFP serves as a reporter, enabling the detection and quantification of promoter activity or the effects of genetic modifications. These cell lines are also employed in drug discovery and development, where the fluorescent signal can be used to screen for compounds that affect specific cellular pathways or target proteins. Additionally, EGFP Stable Cell Lines are valuable in cell sorting techniques, such as fluorescence-activated cell sorting (FACS), where they facilitate the isolation of cells based on the intensity of their fluorescence, aiding in the study of specific cell populations or the development of cell-based therapies.

Supporting Data

EGFP (enhanced green fluorescent protein) fluorescence is used as an indicator to monitor cancer cell response to methotrexate (MTX).(M Dabrowska, et al.,2007)

EGFP (enhanced green fluorescent protein) fluorescence is used as an indicator to monitor cancer cell response to methotrexate (MTX). The study showed that in human colorectal adenocarcinoma C85 cells, the EGFP fluorescence intensity of cells stably expressing EGFP was significantly increased by a factor of 5 after 48 hours of treatment with 1 μM methotrexate. However, there was no significant increase in the overall level of EGFP protein. This enhancement of fluorescence is associated with changes in cell morphology (e.g., increased cell granularity and flattening of cell shape) as well as cell cycle arrest in the G1 phase. The study also found that after methotrexate was removed, the morphology of the cells returned to normal within 10 days.

Influence of 1 μM methotrexate on C85 and pEGFP-C85 cells analyzed by flow cytometry. Changes in cell population pattern in terms of FSC and SSC parameters for C85 and pEGFP-C85 cells, respectively, are presented in A and B. Changes in autofluorescence and EGFP fluorescence in C85 and pEGFP-C85 cells, respectively, are presented in C and D. The histograms of increase in autofluorescence and EGFP fluorescence calculated based on the dot plots for C85 (C) and pEGFP-C85 (D) cells, are presented in E and F.

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