Strain Engineering for Envelope Protein

CD Biosynsis is committed to the production of recombinant viral envelope proteins through the development of prokaryotic and eukaryotic expression systems and to the optimization of microbial fermentation processes and protein purification processes using synthetic biology techniques.

Implications for Synthetic Biology-Driven Envelope Protein Production

Viruses can be classified into non-enveloped and enveloped viruses based on whether or not the outer shell is encased in a lipid-rich envelope. Viral envelope proteins are composed of a double layer of lipids and membrane proteins embedded in them. The enveloped virus enters the host cell through the fusion of the envelope with the cell membrane. Therefore, envelope proteins have an important role in virus adsorption, fusion with host cell membrane and virus assembly. Optimization of the expression conditions of viral envelope proteins using synthetic biology techniques to produce envelope proteins with high concentration and purity is of great value for research in disease diagnosis and prevention.

Figure 1. Schematic view of HBV RNA transcription, envelope protein expression, and genome replication from cccDNA, SphI dimer, and subgenomic expression constructs. (Fu S, et al., 2021)

What We Provide

Deliverable

• Multiple virus envelope proteins.

How We Can Help

Prokaryotic Expression of Virus Envelope Proteins

We are able to use prokaryotes such as E. coli as hosts for viral envelope protein expression, and produce high quality and high purity envelope proteins through processes such as supplemental fermentation and viral envelope protein purification. In order to further improve the production efficiency of the envelope proteins of the prokaryotic expression system, we also work on optimizing the fermentation process and the chromatographic purification process.

• We are able to help our customers select the appropriate medium composition, culture conditions, induction duration, induction agent concentration and other factors affecting the yield of envelope proteins during the fermentation process through experimental design.
• We are committed to developing convenient and effective protein purification processes, and we are currently able to provide protein purification services for immobilized metal affinity chromatography purification.

Expression of Viral Envelope Proteins in Plant Systems

We are able to produce high quality, correctly folded and intact epitopes of envelope proteins using plant expression systems. We provide plasmid construction, agroinfiltration of plants, envelope protein purification and identification services during the expression of viral envelope proteins. We have successfully used plant expression systems for the production of the following viral envelope proteins.

Production of Functionalized Enveloped Virus-Like Particles

Virus-like particles can be applied to the development of long-acting vaccine candidates and targeted drug delivery systems. The incorporation of envelope proteins on the surface of virus-like particles has been reported to improve the efficacy and safety of vaccines. We are committed to producing functionalized enveloped virus-like particles using a bioorthogonal strategy.

Applications of Envelope Protein

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive envelope protein production and meet the needs of customers in a variety of industries.

• For the preparation of vaccines.
• As an effective vaccine candidate.
• As a diagnostic kit antigen.
• For the preparation of lentiviral vectors.
• For the development of virus inhibitors.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in envelope protein production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Fu S, et al. Lost Small Envelope Protein Expression from Naturally Occurring PreS1 Deletion Mutants of Hepatitis B Virus Is Often Accompanied by Increased HBx and Core Protein Expression as Well as Genome Replication. J Virol. 2021 Jun 24; 95(14): e0066021.

Please note that all services are for research use only. Not intended for any clinical use.