EnzyLight™ ADP Assay Kit

Catalog Number: AKBA380

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$1137

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Specifications

Product Information
Product Name	EnzyLight™ ADP Assay Kit
Applications	For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism.
Features	Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ADP can be quantified. Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	Luminescence
Sample Type	Cells etc
Species	All
Assay Time	20 min
Limit of Detection	0.02 µM
Expiration Date	12 months
Description	BioAssay Systems’ ADP Assay Kit provides a rapid method to measure ADP levels. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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