Microtiter Plate Screening (MSP) Technology

Overview Advantages Capabilities FAQS
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Overview

Our company is committed to providing customers with high-quality Microtiter Plate Screening (MSP) technology. Microplate Screening Technology (MSP) is an efficient and accurate screening method that is widely used in the fields of biochemistry, drug development, and enzymology research. By using microplates, a large number of parallel experiments can be performed in extremely small reaction volumes, significantly improving screening throughput and efficiency. Our MSP technology is based on fluorescence or absorbance detection and enables quantitative analysis of target products to ensure accuracy and sensitivity of screening. In addition, we also use automated processing systems to further standardize processes and improve processing capabilities.

Microtiter plate reaction screening and optimization of surfactant chemistry. (Brocklehurst C E, et al., 2018)

This technology is not only suitable for microbial cultivation, but also for various applications such as enzyme activity and metabolite screening.

Our team has rich experience and continues to combine professional knowledge and innovative thinking to provide customized solutions to customers.

Advantages

Microtiter Plate Screening (MSP) technology is a high-throughput screening method that is widely used in microbial culture, enzyme activity detection, and metabolite screening. Microtiter Plate Screening technology plays an important role in modern biological science research due to its high throughput, low cost, quantitative analysis and wide applicability.

High throughput screening

MSP can screen thousands of samples in an instant and can be used to screen large mutant or compound libraries.

Highly Automated

MSP software facilitates automated sample addition and reading that eliminates the need for human intervention and optimizes experiment repeatability.

Precise quantitative analysis

MSP allows precise quantitative analysis by measuring changes in absorbance or fluorescence caused by the substrates/target products on the microplate.

Easy to operate

MSP technology operates fairly intuitively, is suitable for lab daily applications, and can be programmed to minimize human error.

High sensitivity

MSP technology detects target molecules at extremely low concentrations and works well in high sensitivity experiments.

Wide application range

MSP technology can be applied to screen various types of biomolecules and compounds such as enzymes, antibodies, small molecules, drugs, etc.

Strong data processing and analysis capabilities

The data generated by MSP technology can be efficiently processed and analysed by appropriate software, resulting in a fast and effective experimental output.

Cost-effectiveness

MSP technology typically costs less than standard experimental techniques as the amount of reagents is minimal and the time spent in the experiment is short.

Capabilities

Microtiter Plate Screening technology is widely applied because of its high efficiency and precision and is considered one of the key devices in contemporary biological science research and manufacturing. MSP technology has a wide range of application areas, including but not limited to the following aspects:

Screening of enzyme and metabolite mutant libraries

MSP techniques can be used to examine changes in enzyme activity or metabolites. Target products are quantitatively quantified by measuring fluorescence or absorbance changes, making screening accurate and sensitive.

Microbial culture and screening

MSP technology can be applied to the culture and screening of microorganisms, including studying cell heterogeneity, detecting mutants, and growing adherent cells or microbial biofilms. It can also be used to screen microorganisms for bioremediation and biotransformation capabilities.

Improvement of biocatalysts

MSP technology is used to screened and optimize biocatalysts (e.g., improvements in [FeFe] hydrogenases) with potential use in the renewable energy sector.

Immunoassays and clinical analysis

MSP is also widely applied in immunoassays, such as enzyme-linked immunosorbent assays (ELISA) to measure antibodies or antigens in blood.

Bioprocess development

MSP technology is employed to tailor microbial growth regimes and metabolisms in bioprocess development, including optimizing the fermentation phase by measuring biomass, pH and dissolved oxygen.

FAQS

A list of frequently asked questions and answers about Microtiter Plate Screening technology:

Q: What is Microtiter Plate Screening (MSP)?

A: Microtiter Plate Screening (MSP) is a high-throughput microplate-based screening technology. By reacting the microplates and reading them with a reader, it allows you to screen and analyze hundreds of samples in quick time.

Q: What are the key benefits of MSP?

A: The main benefits of MSP technologies are:

  • High throughput: Ability to process multiple samples at the same time, significantly improving experimental efficiency.
  • Accuracy: By detecting changes in absorbance or fluorescence, the target product can be quantitatively analyzed to ensure the accuracy and sensitivity of screening.
  • Flexibility: Suitable for the screening of multiple types of biomolecules and chemical substances, such as enzymes, metabolites, etc.

Q: Where is MSP technology applied?

A: MSP technology is used for a wide variety of applications, not just for these:

  • Drug discovery: Searches for biologically active compounds.
  • Enzyme engineering: Testing and optimizing enzyme activity and stability.
  • Microbial culture: to isolate and cultivate exotic microorganisms and explore their biotransformation properties.

Q: How does MSP technology work?

A: This is how MSP technology generally works:

  • Sample preparation: Prepare the sample to be tested in each microplate well.
  • Chemical or biological reaction: Chemical or biological reactions are reacted under controlled conditions.
  • Test results: Read each well with a microplate reader for changes in absorbance or fluorescence.
  • Analysis of data: Perform quantitative analysis of the test results to exclude samples that don't have the desired features.

Q: What are the drawbacks of MSP technology?

A: While MSP technology is effective, it is not without its limitations:

  • High costs: The high cost of automated equipment and high-throughput instruments may limit the application of small and medium-sized laboratories.
  • Flux limitations: Although there are multiple sizes of microplates (such as 96-well, 384-well, etc.), their screening throughput is still limited and is not suitable for large-scale mutant library screening.

Q: How to maximize the screening potential of MSP technology?

A: Tips on how to make MSP technology screening more efficient include:

  • Automated Equipment: Increase the speed of operations and reduce human error using automated liquid handling systems and automated monoclonal collection systems.
  • Optimize reaction conditions: Control reaction conditions to optimize the rate of reaction and avoid non-specific reactions.
  • Adopt higher throughput

Q: What's the difference between MSP and other screening technologies?

A: In comparison with other screening technologies, MSP has increased throughput and automation. Plate screening, for instance, is simple but not efficient; MSP technology enables rapid and efficient screening through automated machines.

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