Strain Engineering for Erythritol

CD Biosynsis uses our synthetic biology platform to help customers accelerate the production of erythritol. Our scientists are able to help our customers optimize the biosynthesis of erythritol and expand its application areas.

Background

Sweeteners help to reduce sugar intake by reducing the addition of high-calorie sugars to foods. However, regular consumption of early synthetic sweeteners may cause disorders of intestinal flora. Erythritol is a sweetener that is widely found in fruits, vegetables, and fermented foods. Erythritol is largely unmetabolized by the human body, is highly stable, and does not cause intestinal discomfort when consumed. At present, erythritol has been widely used in food, medicine, cosmetics, feed, and chemical products. However, the chemical synthesis method to produce erythritol has the disadvantages of low production efficiency, high cost, and dangerous operation, so it is important to develop and optimize microbial fermentation methods for the industrial production of erythritol.

Figure 1. Metabolic pathway of erythritol biosynthesis. (Nakagawa Y, et al., 2020)

What We Provide

Our scientists are able to help customers solve difficulties in erythritol biosynthesis using tools such as genetic engineering, metabolic engineering, and protein engineering. We are committed to meeting the needs of our customers in multiple fields through our professional and efficient services.

Deliverables

• Erythritol production microorganism.
• Erythritol.

How We Can Help

Development of Synthetic Biology Chassis for Erythritol Production

A variety of microorganisms are capable of producing erythritol from renewable resources. Bacteria use glucose as a substrate to synthesize erythritol, and fungi use glucose and glycerol as substrates to synthesize erythritol. We are able to provide erythritol synthesis services using the following microorganisms as the chassis. If you cannot find your target microorganism in the table below, please contact us directly.

Screening of Strains with High-Level Production of Erythritol

It has been reported in numerous studies that both fungi and bacteria are capable of producing erythritol from some renewable resources, such as glycerol. However, there are differences in the ability of microbial strains to produce erythritol. We can help our customers to achieve high throughput screening of microbial strains with high-level production of erythritol.

Optimization of Culture Conditions

The yield of erythritol is closely related to the culture conditions of the microorganisms. We can help our customers to analyze the effect of different carbon sources, different metal ions, fermentation temperature, and pH on the production of erythritol. We expect to help our customers to improve the yield and efficiency of erythritol production by microorganisms through the optimization of culture conditions.

Mutagenesis of Erythritol-Producing Microorganisms

Mutagenesis of erythritol-producing microorganisms provides a new research direction for improving erythritol production. We are able to use UV light or compounds to induce the production of mutants with enhanced erythritol productivity.

Metabolic Pathway Modification

We are able to inhibit intermediate metabolites in erythritol biosynthesis, increase the efficiency of the pentose phosphate pathway, and inhibit the catabolic pathway of erythritol. We used metabolic engineering tools to achieve targeted modification of the erythritol metabolic pathway.

Applications of Erythritol

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive erythritol production and meet the needs of customers in a variety of industries.

• As a food additive.
• As a special food for people with diabetes and obesity.
• As an antioxidant in vivo, it helps prevent hyperglycemia-induced vascular damage.
• In the field of cosmetics, erythritol can replace part of the role of glycerol.
• Used to make toothpaste.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in erythritol production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Nakagawa Y, et al. Erythritol: Another C4 Platform Chemical in Biomass Refinery. ACS Omega. 2020 Feb 5; 5(6): 2520-2530.

Please note that all services are for research use only. Not intended for any clinical use.