Strain Gene Deletion

Gene deletion is the process of removing one or more genes from an organism's genome. This technique is commonly used in genetic research to study the function of specific genes and their role in various biological processes like strain engineering. Gene deletion can be achieved through several methods, including CRISPR/Cas9 gene editing, homologous recombination, and transposon-mediated mutagenesis.

At CD Biosynsis, we offer a range of services for gene deletion, including custom gene knockout and gene editing services. Our team of expert scientists is dedicated to providing our clients with high-quality gene deletion services in strain engineering that are tailored to their specific research needs.

Fig 1. Gene knockout of P. aeruginosa via homology recombination (Huang W.L, Wilks A. 2017).

Services for Gene Deletion

Our gene deletion services are designed to help researchers study the function of specific genes and their role in various biological processes of strains. We offer several gene deletion services, including:

Custom Gene Knockout

Our custom gene knockout service uses CRISPR/Cas9 technology to delete specific genes from an organism's genome. This technique allows for precise and efficient gene deletion, making it an ideal tool for strain gene function studies.

Gene Editing

In addition to gene knockout, we also offer gene editing services using CRISPR/Cas9 technology to serve for gene deletion.

Transposon-Mediated Mutagenesis

We also offer transposon-mediated mutagenesis services for gene deletion. This technique uses transposable elements to disrupt specific genes in an organism's genome, allowing for the study of gene function and the identification of novel targets for drug development. This method is highlighting the importance of gene deletion in strain engineering for the production of bioactive compounds.

Our Advantages

At CD Biosynsis, we pride ourselves on our commitment to providing our clients with high-quality gene deletion services that are tailored to their specific research needs.

Quality Assurance

Well-established quality system, Quality-by-Design (QbD), and process analytical techniques (PAT) ensure that our gene deletion services are of the highest quality.

Strain Stability and Optimization

Our documentation quality and procedures of strain origin are assessed and approved by a qualified quality assurance service.

Environmental Protection

At CD Biosynsis, we are committed to protecting the environment and providing environmentally friendly services.

Competitive Price and Fast Turnaround Time

Ensure that our clients can complete their research projects on time and within budget.

Technical Support and Guidance

Throughout the gene deletion process, ensuring researchers to achieve their goals.

Gene deletion is a powerful tool for studying gene function and identifying novel targets for drug development. CD Biosynsis offers a range of gene deletion services, including custom gene knockout, gene editing, and transposon-mediated mutagenesis, aiming to expedite scientific research for customers. Contact us today to learn more about our gene deletion services and how we can help you achieve your research goals.

References

1. Fong S.S., Palsson B. Metabolic gene–deletion strains of Escherichia coli evolve to computationally predicted growth phenotypes. Nature Genetics, 2004, 36(10): 1056-1058.
2. Kostner D., Peters B., et al. Importance of codB for new codA-based markerless gene deletion in Gluconobacter strains. Applied Microbiology and Biotechnology, 2013, 97(18): 8341-8349.
3. Chen X.Y, Xu F., et al. Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans. Scientific Reports, 2014, 4(1): 7581.
4. Huang W.L, Wilks A. A rapid seamless method for gene knockout in Pseudomonas aeruginosa. BMC Microbiology, 2017, 17(1): 199.

Please note that all services are for research use only. Not intended for any clinical use.