Strain Gene Editing

Introduction

Synthetic biology is a rapidly growing field that has the potential to revolutionize many industries, including biotechnology, medicine, and agriculture. One of the key tools in synthetic biology is gene editing, which allows scientists to manipulate the DNA of organisms to create new functions, optimize metabolic pathways, and engineer new traits. In recent years, the development of new gene editing tools, such as CRISPR-Cas9, has made gene editing faster, cheaper, and more precise than ever before.

CD Biosynsis is at the forefront of gene editing technology, offering a range of services for strain engineering and synthetic biology.

Figure 1: The mechanisms of genome editing and DSB repair. (Yin, H., Kauffman, K. et al. 2017)

Our Services for Gene Editing

At CD Biosynsis, we offer a wide range of gene editing and synthetic biology services to meet the specific needs of our clients. Our services include:

CRISPR-Cas9 Editing

CRISPR-Cas9 is a versatile and efficient gene editing tool that allows for precise modifications of DNA sequences. Our team has extensive experience in CRISPR-Cas9 editing and can use this tool to create targeted mutations, knockouts, and insertions in a wide range of organisms. For example, we recently used CRISPR-Cas9 to engineer a strain of the bacterium Escherichia coli for the production of resveratrol, a valuable antioxidant compound.

Multiplex Genome Editing by Natural Transformation (MuGENT)

MuGENT is a powerful tool for introducing multiple genetic changes into bacterial genomes simultaneously. This tool allows us to rapidly engineer complex metabolic pathways and optimize the production of valuable compounds, such as biofuels and pharmaceuticals. We have recently developed a new MuGENT system for the bacterium Pseudomonas putida, which has great potential as a platform for sustainable production of biodegradable plastic precursors.

Pathway Engineering

Pathway engineering is a powerful tool for optimizing metabolic pathways in organisms to produce valuable compounds. Our team has extensive experience in pathway engineering and can use this tool to optimize pathways for the production of a wide range of compounds, including biofuels, pharmaceuticals, and specialty chemicals. For example, we recently engineered a pathway in the yeast Saccharomyces cerevisiae for the production of beta-carotene, a valuable pigment and antioxidant.

Metabolic Engineering

Metabolic engineering is a powerful tool for optimizing the metabolism of organisms for specific applications. Our team has extensive experience in metabolic engineering and can use this tool to optimize the production of a wide range of compounds, including biofuels, pharmaceuticals, and specialty chemicals. For example, we recently engineered the bacterium Rhodobacter sphaeroides for the production of hydrogen gas, a promising renewable energy source.

Synthetic Biology

Synthetic biology is a rapidly growing field that combines engineering principles with biology to design and build new biological systems. Our team has extensive experience in synthetic biology and can use this tool to engineer new organisms for specific applications.

Genome Synthesis

Genome synthesis is a powerful tool for creating new organisms with customized genomes. Our team has the expertise and technology to synthesize entire genomes or large gene clusters, enabling the creation of organisms with novel functions and capabilities. For example, we are currently working on the synthesis of a new genome for the bacterium Vibrio natriegens, which has great potential as a platform for synthetic biology and biotechnology.

Our Advantages

• Expertise, quality, and efficiency.
  Our team of experts has years of experience in gene editing and strain engineering, and we are committed to delivering the highest quality results for our clients. We use the latest tools and techniques to ensure that our gene editing services are precise, efficient, and cost-effective.
• Usage of Vibrio natriegens, reduced turnaround time.
  A fast-growing bacterium that has become increasingly popular in synthetic biology. V. natriegens has a doubling time of just 10 minutes, which makes it one of the fastest-growing organisms on the planet. This allows us to rapidly engineer new strains and optimize metabolic pathways, reducing the time and cost of development.
• Exceptional customer service and support.
  We work closely with our clients to understand their specific needs and develop customized solutions that meet their goals. Our team is available to answer any questions and provide ongoing support throughout the project.

Conclusion

Gene editing is a powerful tool for strain engineering and synthetic biology, and at CD Biosynsis, we are committed to providing the highest quality services for our clients. Our team of experts has years of experience in gene editing and strain engineering, and we use the latest tools and techniques to ensure that our services are precise, efficient, and cost-effective. Whether you are developing new strains for biotechnology, agriculture, or medicine, we can help you achieve your goals and drive innovation in your industry. Contact us today to learn more about our services and how we can help you achieve your goals.

References

1. Yin, H., Kauffman, K., et al. Delivery technologies for genome editing. Nat Rev Drug Discov 16, 387–399 (2017). https://doi.org/10.1038/nrd.2016.280.
2. Ng, I., Keskin, B. B., et al. A Critical Review of Genome Editing and Synthetic Biology Applications in Metabolic Engineering of Microalgae and Cyanobacteria. Biotechnology Journal. 2020, 15, 1900228. https://doi.org/10.1002/biot.201900228
3. Zhang, X., Lin, Y., et al. Synthetic biology and genome-editing tools for improving pha metabolic engineering. Trends Biotechnology (7), 2020.

Please note that all services are for research use only. Not intended for any clinical use.