Strain Gene Insertion

Genetic engineering has long been a challenge for medical science. However, with the advent of gene editing technologies, CD Biosynsis is now on the brink of the next era of medicine. Gene insertion, one of the techniques of genetic engineering, holds great promise for a wide range of applications, including strain engineering.

Science Behind Gene Insertion

Gene insertion, also known as targeted integration, is a type of gene editing that involves adding a new gene to a specific location in the genome. This is achieved through the use of nucleases, enzymes that cut DNA at specific locations. The cut DNA is then repaired using a DNA template that contains the desired gene. This results in the insertion of the new gene into the genome.

Gene insertion has the potential to revolutionize research with strains. Rather than correcting individual mutations, gene insertion can theoretically correct virtually all mutations in a gene. As a result, it is a highly versatile and powerful tool for developing new drugs based on the genetic component.

Fig. 1. cDNA insertion into the plasmid and the transformation of the E-coli the general steps. (Saifuddin N.M, 2001)

Services for Gene Insertion

CD Biosynsis is at the forefront of gene insertion technology, offering a range of services for gene insertion. The team of experts has extensive experience in the design and production of gene insertion vectors, as well as the use of nucleases for targeted integration.

Gene Insertion Vector Design

The design of an effective gene insertion vector is critical for the success of targeted integration. CD Biosynsis uses state-of-the-art software and algorithms to design vectors that are highly specific and efficient. Our team has experience designing vectors for a wide range of applications, including the correction of CFTR mutations in cystic fibrosis.

Nuclease-Mediated Gene Insertion

CD Biosynsis uses nucleases such as CRISPR/Cas9 and TALENs to achieve targeted integration. Our team has extensive experience in the use of these nucleases and can optimize their use for each individual application.

Ex Vivo Gene Insertion

CD Biosynsis offers ex vivo gene insertion services, which involve the modification of cells outside the body before they are transplanted back into the patient. This approach has been shown to be effective for the treatment of genetic disorders such as sickle cell anemia.

In Vivo Gene Insertion

CD Biosynsis is also developing in vivo gene insertion technologies, which involve the direct modification of cells within the body. This approach has the potential to be highly effective for the treatment of genetic disorders such as Huntington's disease.

Customized Solutions

We understand that each application is unique and requires a customized solution. That's why we work closely with our clients to understand their specific needs and develop customized solutions that meet their requirements. For more information about customized services please contact us.

Our Advantages

CD Biosynsis has several advantages over other companies in the field of gene insertion.

Expertise

Our team of experts has years of experience in the field of gene editing and gene therapy. We have extensive experience in the design and production of gene insertion vectors, as well as the use of nucleases for targeted integration.

State-of-the-Art Facilities

CD Biosynsis has state-of-the-art facilities, which are designed to meet the highest standards of quality and safety.

Commitment to Quality

CD Biosynsis uses rigorous quality control processes to ensure that our products and services meet the highest standards of safety and efficacy.

Customization

We work closely with clients to understand their specific needs and develop customized solutions that meet their requirements.

Gene insertion holds great promise for strain engineering. CD Biosynsis is at the forefront of gene insertion technology, offering a range of services for the treatment of genetic disorders. With our state-of-the-art facilities, commitment to quality, and customized solutions, CD Biosynsis is the ideal partner for gene insertion therapies and feel free to contact us for more information.

References

1. Phan Q.V, Contzen Jörg, et al. Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease. Scientific Reports, 2017, 7(1): 17771.
2. Saifuddin N.M. Cloning and Sequencing of Glutathione S-Transferase From Hevea brasiliensis, 2001.

Please note that all services are for research use only. Not intended for any clinical use.