Gene knockout/knockdown Services

Overview Capabilities Advantages Services Process FAQs

Overview

CD Biosynsis is providing Gene Knockout and Gene Knockdown. We operate our primary business on leading-edge CRISPR/Cas9 technology, which can knock out or suppress genes permanently through precision genome editing to offer customers a complete gene function research product.

Our gene knockout services provide different vector-based double strand break technologies (CRISPR/Cas9 vectors), TALENs and ZFNs vector-based gene editing, to knock out the target genes. Also, we offer genome-wide knockout screening and services like double gene knockouts for certain genes like MC3R and MC4R.

In the case of gene knockdown services, we employ RNA interference methods like siRNA and Morpholino oligonucleotides to reduce the expression level of the target gene by specifically binding to its target gene's mRNA or DNA sequence. It's a short-term approach that can also lead to gene function research in the long run, by developing cell lines with stably expressed knockdown genes.

We work with many cell lines and animals models, such as rats, mice and zebras, and can cater to all kinds of research needs. With these technologies, we equip customers with deep insights into genes involved in normal physiology and disease, which are the building blocks of new drugs and gene therapy.

Essentially, based on the technical expertise and years of experience in gene editing projects, we have pledged to deliver customers the high quality and efficient gene knockout and knockdown services to support the innovation of scientific research and drug discovery.

Capabilities

The Gene knockout/knockdown Services provided by our company mainly include the following sub-services:

Service type	Service content	Technical characteristics	Turnaround	Verification method
Gene knockout services	mono-allelic knockout	CRISPR/Cas9, TALEN and other technologies	3-7 months	Sequencing identification, WB verification
	multiple gene knock down	CRISPR/Cas9, TALEN and other technologies	3-7 months	sequencing
	Large gene knockout	CRISPR/Cas9, TALEN and other technologies	3-7 months	sequencing
	Cell line gene knockout	CRISPR/Cas9, TALEN and other technologies	3-7 months	Sequencing identification, WB verification
	Animal model gene knockout	CRISPR/Cas9, TALEN and other technologies	6-15 months	PCR and sequencing
Gene knockdown services	RNAi gene knockdown	shRNA, siRNA and other technologies	10-12 weeks	RT-qPCR, FACS detection
Gene knockdown services	CRISPRi gene knockdown	CRISPRi technology	12-16 weeks	RT-qPCR, FACS detection

The exact time may vary based on the complexity of the project and customer needs.

Advantages

Gene knockout/knockdown services have the following advantages:

High Efficiency

Gene knockouts are quick and precise ways to test gene function. In comparison with RNA interference, its information is more reliable and it is uninterfering.

Accuracy

With gene knockout technology, scientists can learn the function of specific genes in real time and what their roles are in normal development, physiology and disease.

Stability

Correct gene change and stable effect, good for complex gene knockouts.

High efficiency

Recent gene editing technologies like CRISPR-Cas9 cut gene knockouts by up to 20%, reducing gene knockout times to several months.

Extensive applications

Gene knockout is widely deployed in drug discovery, preclinical trials, etc. It can cater to the customers' requirement of screening, optimization/development and experiment analysis on multiple levels.

Technical advantage

Newer gene targeting approaches like TurboKnockout technology, while keeping stable targeting effect of ES, shorten the breeding process and boost the efficacy of research compared to current technologies.

Services Process

The following is the service process of gene knockdown/knockdown services:

Signing of Contract

Once company receives customer requirement, immediately notified to the customer with technical proposal. Having settled the technical service plan with customer, it submits the quotation, talks to customer about price and service information and enters into the contract.

Project implementation

gRNA design and synthesis: Gene Design and Synthesised on the gene data we receive from our customers (gene name, NCBI, DNA Sequence).
Construction of vectors: Add gRNA and Cas9 enzyme sequences to vectors to construct the plasmid or viral vector that is needed to edit genes.
Transfection: Choose the desired transfection protocol (eg chemical transfection protocol, electrotransfection protocol, viral protocol) for introducing the created vector into the cells.
Monoclonal screening: Monoclonal cell lines with knockdown or knockdown of the target genes are screened by FACS sorting or other.

Project delivery

Confirm positive clones: Perform PCR identification and sequencing of single clones that were screened to verify gene knockdown/knockdown success.
Project Reporting: Prepare project reports containing the sgRNA sequences, experimental data, sequencing data and analysis, etc for customers' future demonstration material requests.
Product distribution: Manage the shipment of positive cloned cell lines and the shipment of relevant data reports as per the customers need.

After-sales service

Provide technical follow-up assistance (Technical reports, Genotype testing procedures, Cell Culture Protocols etc.).
Offer other experimental testing support if necessary.

Signing of Contract

Project implementation

Project delivery

After-sales service

By this above procedure, the company can provide customers with a one-stop services starting from gene knockout/knockout strategy to the delivery of final positive clone to enable the project to be implemented smoothly and completed with good quality.

FAQs

Q: What are gene knockouts and gene knockdowns?

A: Gene knockout describes a genetic condition where a gene has been shut down, technical process involving deletion or substitution of DNA sequences. Gene knockdown: the process of modifying a gene expression by, for example, RNA interference to silence it, without eliminating its activity.

Q: What is the difference between gene knockdown and gene knockdown?

A: Gene knockouts are typically irreversible, ie, the target gene is never active again; gene knockdowns are merely reactive, reducing gene activity through RNA interference and other mechanisms, but not completely destroying gene function.

Q: How to choose the right gene knockout procedure?

A: Gene knockout approaches can be chosen based on research requirements. CRISPR/Cas9, for instance, is a generic gene knockout technology that works in many biological systems.

Q: What is the quality control of cell lines that have been knocked out?

A: The knockout cell line must be genotyped and phenotyped extensively to satisfy the knockout function.

Q: Can gene knockout be used for multiple species?

A: In fact, gene knockouts can be used in many species, like mice, rats, etc., to learn how genes work and what their roles are in organisms.

Q: How do I get double allelic knockouts?

A: We have to do both allelic knockouts by homologous directed repair (HDR) technology and make sure the CRISPR/Cas9 mechanism is able to perform simultaneous activity against both alleles.

Q: Are gene knockout services customized?

A: Yes, we offer custom gene knockout services which can customize and deliver custom gene knockout products according to customer 's needs.

Q: Can gene knockout be used to build rare disease models?

A: Sure, we can create many different forms of genetically engineered mouse models — such as gene knockout mice — quickly to study rare diseases and screen drugs.

Q: What is the turnaround time for gene knockout and gene knockdown services?

A: The turnaround time for gene knockout and gene knockdown services varies depending on several factors, including the complexity of the experimental design, the number of cell lines or animal models required, and the speed of experiment execution. Typically, from the time a customer places an order to the completion of the experiment and delivery of results, the entire process can take anywhere from a few weeks to several months.

Q: Where does gene knockouts and gene knockdowns get used in the lab?

A: Gene knockouts and gene knockdowns are commonly used in functional genomics studies to find out how genes operate in living organisms. If we for instance knock out/knock down certain genes, how the genes are involved in disease formation, cell development, etc. can be studied.

Please note that all services are for research use only. Not intended for any clinical use.

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