Strain Gene Overexpression

Gene overexpression is a fundamental tool in synthetic biology that allows for the efficient production of proteins for a wide range of applications, including biopharmaceuticals, industrial enzymes, and biofuels. Here in CD Biosynsis, we explore the role of gene overexpression in strain engineering from a synthetic biology perspective and how we can help in optimizing gene overexpression for diverse applications.

Introduction

The ability to overexpress genes in microorganisms has revolutionized the field of synthetic biology. Gene overexpression involves the production of large quantities of target proteins by increasing the transcription and translation of the gene of interest. This technique has been applied in the engineering of microorganisms to produce a wide range of products, including biofuels, chemicals, and biopharmaceuticals.

Strain engineering, on the other hand, involves modifying microorganisms to improve their performance in various processes. This can be achieved by introducing genetic modifications that result in improved growth, productivity, and stability.

Fig 1. Schematic illustration of gene overexpression for improving resveratrol production in S. cerevisiae. (Liu, D., Li, B., et al. 2017)

Our Services for Gene Overexpression

At CD Biosynsis, we offer a comprehensive range of services to optimize gene overexpression for diverse applications. Our expert team employs advanced technologies and methodologies to ensure reliable and efficient gene expression.

We offer gene synthesis services that involve the design and synthesis of genes optimized for expression in specific host organisms. Our team can

-Modify the codon usage,

-Remove destabilizing sequences,

-Add regulatory elements to enhance expression.

The target expression level of the gene is achieved through the modification and design of the gene.

Our cloning services allow the synthesized genes to be cloned into various expression vectors to achieve high levels of gene expression. We use state-of-the-art cloning techniques to ensure reliable and efficient gene cloning.

- Restriction enzyme digestion and DNA ligation

- Polymerase Chain Reaction (PCR) amplification

- Plasmid-mediated transformation

- Gene transfection

Our gene optimization services involve the identification and removal of regulatory elements that may reduce gene expression levels. This can be achieved by modifying

- promoter,

- ribosome binding site,

- other regulatory elements,

to obtain optimal gene expression levels.

We offer protein expression services that involve the production of target proteins using optimized gene expression. Our team can produce proteins at a large scale with high purity and quality.

Our Advantages

At CD Biosynsis, we have a competitive advantage in the field of synthetic biology. Our team of experts has extensive experience in gene overexpression, strain engineering, and protein expression optimization. We use cutting-edge technologies and methodologies to ensure reliable and efficient gene overexpression.

• Host Organism Flexibility
  We have ability to optimize gene expression in a wide range of host organisms like various bacterial, fungal, and yeast strains, among others.
• Customized Solutions
  We can develop customized solutions for each client's specific needs.
• Quality Control
  We are committed to quality and use rigorous quality control measures to ensure that our products and services meet the highest standards.
• Cutting-edge Technologies
  We use cutting-edge technologies and methodologies to ensure reliable and efficient gene overexpression.

CD Biosynsis offers a range of advantages in the field of synthetic biology, including host organism flexibility, customized solutions, quality control, and state-of-the-art technologies. Our team of experts has extensive experience in gene overexpression, strain engineering, and protein expression optimization, and can provide innovative solutions for diverse applications.

CD Biosynsis is the ideal partner to work with for accelerating your research and feel free to contact us.

References

1. Prelich G. Gene overexpression: uses, mechanisms, and interpretation. Genetics. 2012 Mar;190(3):841-54. doi: 10.1534/genetics.111.136911.
2. Cao, G., Zhang, P., et al. Gene Overexpression in Streptomyces hygroscopicus Associated with DNA Amplification. Current Microbiology 74, 979–986 (2017). https://doi.org/10.1007/s00284-017-1278-y.
3. Liu, D., Li, B., et al. Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae. Frontiers of Chemical Science and Engineering. 11, 117–125 (2017). https://doi.org/10.1007/s11705-016-1601-3.

Please note that all services are for research use only. Not intended for any clinical use.