Strain Gene Regulatory Element Modification

Introduction

Gene regulatory elements (GREs) play a critical role in gene expression by controlling the activity of promoters and enhancers in the genome. Synthetic biology approaches have enabled the modification of GREs to achieve desired gene expression patterns and optimize the performance of biological systems. CD Biosynsis has put much effort on gene regulatory element modification research and can help you achieve your goals in strain engineering.

Figure 1: Operon genome science educational vector illustration.

Services We Offer

Gene regulatory element modification is a critical tool for optimizing gene expression and achieving desired outcomes in synthetic biology and biopharmaceuticals. CD Biosynsis offers a range of gene regulatory element modification services.

Promoter Engineering

Promoters are GREs that control the initiation of transcription. CD Biosynsis can modify promoter sequences to optimize gene expression levels, temporal expression patterns, and tissue-specific expression.

Enhancer Engineering

Enhancers are GREs that control the level and specificity of gene expression. CD Biosynsis can engineer enhancers to achieve desired gene expression patterns, including tissue-specific expression, inducible expression, and conditional expression.

Transcription Factor Binding Sites (TFBS) Engineering

TFBSs are GREs that control the binding of transcription factors to DNA, which regulates gene expression. CD Biosynsis can engineer TFBSs to achieve specific transcription factor binding and optimize gene expression.

CRISPR/Cas9-based Gene Editing

CD Biosynsis uses CRISPR/Cas9-based gene editing to modify GREs with high precision and efficiency. This technology allows us to create precise modifications to GREs to achieve the desired gene expression patterns.

Our Services Characterization

Our team of experts includes experienced molecular biologists, bioinformaticians, and genetic engineers who work collaboratively to provide high-quality gene regulatory element modification services.

High Precision

We use state-of-the-art gene editing technologies, including CRISPR/Cas9-based gene editing, to achieve precise modifications to GREs.

Customized Solutions

Our services are flexible and can be tailored to meet the unique needs of each project.

Cost-Effective and Fast Turnaround Times

We understand the importance of timely delivery in the biotechnology industry. Our services are designed to provide fast turnaround times and cost-effective services without compromising on quality.

Conclusion

Gene regulatory element modification is a powerful tool for optimizing gene expression and achieving desired phenotypic outcomes in synthetic biology and strain engineering. CD Biosynsis offers a range of services for gene regulatory element modification, including promoter engineering, enhancer engineering, TFBS engineering, and CRISPR/Cas9-based gene editing. Contact us today to learn more about how we can help you achieve your goals in strain engineering.

References

1. Yao, D., Han, X. et al. Enhanced extracellular production of raw starch-degrading α-amylase in Bacillus subtilis through expression regulatory element modification and fermentation optimization. Microbial Cell Factories 22, 118 (2023). https://doi.org/10.1186/s12934-023-02116-z.
2. Carelli, F.N., Liechti, A. et al. Repurposing of promoters and enhancers during mammalian evolution. Nature Communications 9, 4066 (2018). https://doi.org/10.1038/s41467-018-06544-z
3. Erqing, J., Lynn, W., Yun, J. et al. (2017). Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression. Synthetic and Systems Biotechnology 2(4), 295-301 (2017). https://doi.org/10.1016/j.synbio.2017.10.003.

Please note that all services are for research use only. Not intended for any clinical use.