Gene Synthesis Service

Overview Capabilities Advantages Services Process FAQs

Overview

Our Gene Synthesis Services use cutting edge technologies and codon optimization technologies to generate genes of all lengths and complexity. We manage everything from designing gene sequences and creating oligonucleotides, to PCR amplification, cloning and sequencing for the precision and efficacy of the gene synthesis.

Our gene synthesis services are not only applicable for fundamental studies, but they are also commonly utilized for drug discovery, vaccine development, and genetic engineering. Our experience covers genes that contain GCs, repetitive sequences, and special structures. Moreover, we also provide value-added services including customized vector assembly, plasmid assembly and mutant assembly to cater customers' diverse requirements in gene function research and biotechnology fields.

We provide precision, delivery time, and reasonable pricing for gene synthesis services, and we strive to deliver the most reliable and all-encompassing solution to our customers. Whether you require gene synthesis for short or long time, we will provide high quality services and support to ensure the success of your scientific research and development.

Capabilities

The following is a breakdown of the gene synthesis services we provide:

Type	Gene length	Characteristics	Application
standard gene synthesis	≤ 10 kb	Standard services for most application scenarios	life science research
Large fragments and genes	10-200 kb	Using yeast in vivo assembly technology	synthetic genome
DNA fragment synthesis	≤ 1.2 kb	Simple genetic construction and modification	plasmid construction
Rapid gene synthesis	≤ 1.2 kb	Fast track, timely delivery	Time-sensitive projects
pETgene gene synthesis	≤ 3 kb	Deeply optimized sequences for prokaryotic expression	prokaryotic expression
long single-stranded DNA synthesis	≤ 4 kb	Low cytotoxicity, high editing efficiency	CRISPR Knock-in
mRNA poly(A) gene synthesis	≤ 5 kb	Contains up to 120 poly(A) sequences	mRNA vaccine related
total gene synthesis	unlimited length	Can be cloned into designated vectors and provide a variety of additional services	Gene mutation/recombination, efficient expression, synthesis of cDNA, etc.
High-throughput gene synthesis	unlimited number	Synthesis of hundreds to nearly 10,000 genes at one time	Large-scale gene synthesis

Advantages

The Gene Synthesis Service we provide is a one-stop service that covers the entire process from gene design to synthesis.

Gene sequence design and optimization

Provide free consulting services such as gene structure design, codon optimization and gene cloning and expression scheme design.
Use professional software such as GENEius for sequence optimization to prevent duplicate sequences and promote GC content distribution.

Gene synthesis

Design and synthesize the target gene based on the gene sequence information provided by the customer.
The synthesized genes range in length, ranging from less than 200bp to more than 10kb.
Providing multiple vector options, customers can choose to clone genes into designated or universal vectors.

Delivery and support

Provide complete experimental materials including plasmids, strains, and sequencing maps.
Provide detailed experimental reports, including plasmids loaded with target genes, bacterial strains, sequencing maps/sequences, etc.
Provide electronic data, such as PDF version of experimental reports, sequencing color waveform charts, base sequences and plasmid maps.

Technical support and service process

Customers only need to provide the required gene sequences, and the company will conduct feasibility analysis and design synthesis plans based on customer needs.
The company has a top technical team and rich experience, and can complete gene synthesis quickly and accurately.
Provide timely progress inquiries and after-sales services to ensure that customers receive adequate support throughout the project process.

Quality control and verification

All synthesized genes are verified by sequencing to ensure sequence accuracy.
Possible error sites are repaired by site-directed mutations and verified by sequencing again.

Confidentiality and Security

Customer data and genetic sequences are completely confidential to ensure the safety of customers 'intellectual property rights.

Price and cycle

Depending on the length of the gene and service content, the price starts from 500 yuan/gene, and the cycle ranges from 5 working days to 25 working days.

Through the above one-stop services, we can provide customers with efficient and high-quality gene synthesis services to meet their various needs in the fields of gene research, disease diagnosis, treatment and prevention.

Services Process

The following is the service process for gene synthesis services. Through these processes, we ensure that we provide customers with high-quality and accurate gene synthesis services.

Customers submit gene sequences

Customers first need to provide the required gene sequence information, including detailed requirements such as restriction enzyme cleavage sites and vector selection.

Scheme design and confirmation

After receiving the gene sequence provided by the customer, the company will conduct software analysis to check whether the gene contains repetitive sequences and special structures, and design a gene synthesis plan according to specific circumstances.
After the design is completed, the plan will be forwarded to the customer for confirmation.

Signing contract

After the customer confirms the plan, both parties sign a gene synthesis service agreement and confidentiality contract. Customers need to pay 50% of the synthesis fee in advance before the company can start synthesis work.

Gene synthesis

Design an Oligo synthesis scheme and carry out chemical synthesis of single strands on a fully automatic synthesis instrument.
Using OVER-LAP PCR technology, the single-stranded Oligo is spliced into a double-stranded full-length gene.
The PCR products were digested, purified, ligated into plasmids, and the plasmids were transferred into cell strains for culture.

Quality control

Positive clones were extracted for DNA sequencing to confirm the accuracy of the inserted fragments.
Possible erroneous sites were corrected by site-directed mutation and sequenced again.

Reporting and Delivery

Provide experimental reports, including plasmids loaded with target genes, bacterial strains, sequencing maps/sequences, etc.
After the synthesis is completed, the company informs the customer and starts shipping after receiving the full payment.

Customers submit gene sequences

Scheme design and confirmation

Signing contract

Gene synthesis

Quality control

Reporting and Delivery

FAQs

In order to help customers better understand the gene synthesis services our company provides, the following are common questions and answers:

Q: What is gene synthesis?

A: Gene synthesis: it involves creating genes without a template in vitro. This conserves time and money while optimizing DNA and optimising protein expression.

Q: What is the difference between gene synthesis and molecular cloning?

A: Gene synthesis is different from molecular cloning because it doesn't involve complex steps like cloning strategy development, primer design, PCR, etc. These steps take time and effort. Furthermore, gene synthesis is typically less expensive and more convenient than traditional cloning in that it doesn't need physical templates or the design limitations of cloning.

Q: What are the advantages of gene synthesis services?

A: The main advantages of gene synthesis services include time savings, cost reductions, enhanced DNA performance, and convenience. In addition, gene synthesis can also be codon optimized to improve the efficiency of protein expression.

Q: How can I contact your company for gene synthesis services?

A: You can contact directly through a salesperson or technical department to provide detailed background information and experimental requirements. The technician will negotiate the experimental plan with you and inform you of the specific service price and completion time.

Q: What are your company's specific requirements for experimental materials?

Q: What types of genes can your company synthesize?

Q: How does your company optimize the genes you synthesize?

Q: How are synthetic genes delivered?

A: We provide complete delivery standards including lyophilized plasmid DNA, recombinant plasmid vectors, sequencing peak maps, COA documents and gene sequence comparison documents.

Q: What if you find a mistake in the synthesized gene?

A: If the sequencing results find erroneous sites, we will adopt corresponding technical measures to repair and proofread them.

Q: Does your company provide free design services?

A: Yes, we provide free consulting services such as gene structure design, codon optimization and cloning expression scheme design.

Q: How does your company ensure the confidentiality of customers 'data and genetic sequences?

A: We keep our customers 'data and genetic sequences completely confidential to ensure the rights and interests of research owners.

Q: What is your company's service process?

A: The customer provides the gene sequence information needed for synthesis, and we conduct software analysis and design the synthesis scheme, then start synthesis and finally pass sequencing verification.

Please note that all services are for research use only. Not intended for any clinical use.

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