HEK Cells Genetic Modification

Introduction

HEK cells are widely used in the biotechnology industry for the production of recombinant proteins, vaccines, and viral vectors. The genetic modification of HEK cells can enhance their productivity, stability, and functionality, making them ideal candidates for large-scale production of biologics.Genetic manipulation of HEK cells involves the insertion, deletion, or modification of specific genes to achieve a desired phenotype. This process can be achieved using various genetic engineering techniques, including CRISPR/Cas9, TALENs, and ZFNs.

CD Biosynsis is a biotechnology company that specializes in genetic manipulation of cells and organisms. We offer a range of services for genetic manipulation of cells.

Figure 1: Engineering the HEK293 cell line for improved culture performance. (Abaandou L., et al. 2021)

Our Services for Genetic Manipulation of HEK Cells Modification

 Cell Line Development

Our cell line development services aim to generate high-producing, stable cell lines for the production of recombinant proteins, viral vectors, and vaccines. We offer customized solutions for each project, including vector construction, transfection optimization, and high-throughput screening. Our team of experts can develop stable cell lines that meet the specific requirements for each project, including cell growth, viability, stability, and productivity.

• Vector construction
• Transfection optimization
• High-throughput screening
• Stable cell line development
• Customized solutions for each project

Genome Editing

We use the CRISPR/Cas9 system for precise and efficient genome editing of HEK cells. Our genome editing services include gene knockout, knock-in, and point mutation. We can insert, delete, or modify specific genes to achieve the desired phenotype, such as increased productivity or enhanced stability. Our services include design and construction of guide RNAs and donor templates, transfection optimization, and screening of edited cell lines.

• CRISPR/Cas9 system
• Gene knockout, knock-in, and point mutation
• Guide RNA and donor template design and construction
• Transfection optimization
• Screening of edited cell lines

Cell Banking

We provide comprehensive cell banking services for the long-term storage and maintenance of our clients' cell lines. Our state-of-the-art facilities and strict quality control measures ensure the highest quality of our cell banks. We offer both master and working cell banks, with detailed documentation and quality control testing for each cell bank.

• Comprehensive cell banking services
• Long-term storage and maintenance of cell lines
• Master and working cell banks
• Detailed documentation and quality control testing

Process Development

We offer process development services for the optimization of upstream and downstream processes for the large-scale production of biologics. Our team of experts can develop customized processes tailored to meet the specific requirements of each project. Our services include process optimization, scale-up, and technology transfer. We use state-of-the-art technologies, including bioreactors, chromatography systems, and filtration systems, to develop efficient and cost-effective processes for the large-scale production of biologics.

• Upstream and downstream process optimization
• Customized solutions tailored to each project
• Process optimization, scale-up, and technology transfer
• Use of state-of-the-art technologies, including bioreactors, chromatography systems, and filtration systems

Our Advantages

• High-Throughput Screening
• CRISPR/Cas9 Genome Editing
• Quality Control
• Expertise

CD Biosynsis provides a wide range of services for genetic manipulation of HEK cells modification, including cell line development, genome editing, cell banking, and process development. Our services are designed to meet the specific needs of our clients and provide customized solutions for their research and development goals. Our team of experts has extensive experience in the biotechnology industry and uses state-of-the-art technologies to provide efficient and reliable services for our clients. Contact us today to learn more about our services and how we can help you achieve your research and development goals.

References

1. Abaandou L., et al. Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes. Cells, 2021, 10(7): 1667.
2. Sun Z., et al. Effects of NSUN2 deficiency on the mRNA 5-methylcytosine modification and gene expression profile in HEK293 cells. Epigenomics, 2019, 11(4): 439-453.

Please note that all services are for research use only. Not intended for any clinical use.