GeniePlex Human CD8+ T Cell Related Cytokines, 16-plex (96 Tests)

Catalog Number: AKME009

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Specifications

Product Information
Product Name	GeniePlex Human CD8+ T Cell Related Cytokines, 16-plex (96 Tests)
Analytes	GM-CSF Granzyme B IFN-Gamma IL-2 IL-4. IL-5 IL-6 IL-10 IL-13 IL-15 MIP-1-Alpha MIP-1-Beta RANTES sCD137 sFasL TNF-Alpha
Reactivity	Human
Sensitivity	GM-CSF: < 5 pg/mL, Granzyme B: < 10 pg/mL, IFN-Gamma: < 3 pg/mL, IL-2: < 2 pg/mL, IL-4, < 1 pg/mL, IL-5: < 2 pg/mL, IL-6: < 5 pg/mL, IL-10: < 2 pg/mL, IL-13: < 5 pg/mL, IL-15: < 5 pg/mL, MIP-1-Alpha: < 2 pg/mL, MIP-1-Beta: < 5 pg/mL, RANTES, < 5 pg/mL, sCD137, < 5 pg/mL, sFasL: < 2 pg/mL, TNF-Alpha, < 1 pg/mL
Detection Method	Flow Cytometry
Assay Time	2 hours
1x Ab-conjugated beads (premixed)	S4P2 - anti-human MIP-1-Alpha, S4P3 - anti-human TNF-Alpha, S4P6 - anti-human sCD137, S4P7 - anti-human IL-2, S4P8 - anti-human MIP-1-Beta, S4P9 - anti-human IFN-Gamma, S4P10 - anti-human Granzyme B, S4P11 - anti-human IL-15, S5P2 - anti-human sFasL, S5P3 - anti-human GM-CSF, S5P4 - anti-human IL-4, S5P5 - anti-human IL-5, S5P6 - anti-human IL-6, S5P7 - anti-human RANTES, S5P10 - anti-human IL-10, S5P12 - anti-human IL-13
2x Biotin-detection Ab (premixed)	Biotin - anti-human MIP-1-Alpha, Biotin - anti-human TNF-Alpha, Biotin - anti-human sCD137, Biotin - anti-human IL-2, Biotin - anti-human MIP-1-Beta, Biotin - anti -human IFN-Gamma, Biotin - anti-human Granzyme B, Biotin - anti-human IL-15, Biotin - anti-human sFasL, Biotin - anti-human GM-CSF, Biotin - anti-human IL-4, Biotin - anti-human IL-5, Biotin - anti-human IL-6, Biotin - anti-human RANTES, Biotin - anti-human IL-10, Biotin - anti-human IL-13
Buffers	2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix	Lyophilized Standard Mix- Human CD8+ T Cell Related Cytokines, 16-plex. One vial containing lyophilized recombinant GM-CSF, Granzyme B, IFN-Gamma, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1-Alpha, MIP-1 Beta, RANTES, sCD137, sFasL, TNF-Alpha.
LLOQs	GM-CSF: <10 pg/mL, Granzyme B: <20 pg/mL, IFN-Gamma: <10 pg/mL, IL-2: <10 pg/mL, IL-4: <3 pg/mL, IL-5: <5 pg/mL, IL-6: <10 pg/mL, IL-10: <5 pg/mL, IL-13: <10 pg/mL, IL-15: <10 pg/mL, MIP-1-Alpha: <5 pg/mL, MIP-1-Beta: <10 pg/mL, RANTES: <10 pg/mL, sCD137: <10 pg/mL, sFasL: <5 pg/mL, TNF-Alpha: <2 pg/mL.
ULOQs	GM-CSF: > 5,000 pg/mL, Granzyme B: > 5,000 pg/mL, IFN-Gamma: > 2,000 pg/mL, IL-2: > 2,000 pg/mL, IL-4: > 1,000 pg/mL, IL-5: > 2,000 pg/mL, IL-6: > 5,000 pg/mL, IL-10: > 5,000 pg/mL, IL-13: > 5,000 pg/mL, IL-15: > 5,000 pg/mL, MIP-1-Alpha: > 2,000 pg/mL, MIP-1-Beta: > 5,000 pg/mL, RANTES: > 5,000 pg/mL, sCD137: > 5,000 pg/mL, sFasL: > 2,000 pg/mL, TNF-Alpha: >1,000 pg/mL.
Standard dose recovery	70-130%
Intra-assay CV	Intra-assay CV: <10%
Inter-assay CV	Inter-assay CV: <20%
Sample volume	15 µL/test

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