GeniePlex Human Cytokine 16-Plex Panel 2 (96 tests)

Catalog Number: AKME012

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Specifications

Product Information
Product Name	GeniePlex Human Cytokine 16-Plex Panel 2 (96 tests)
Analytes	CXCL2 GM-CSF IFNalpha2 IFN? IL-1beta IL-1RA IL-4 IL-6 IL-10 IL-12p70 IL-17A IL-18 IL-21 IL-22 MCP-1 TNFalpha
Reactivity	Human
Sensitivity	CXCL2: < 10 pg/mL, GM-CSF: < 5 pg/mL, IFNalpha2: < 5 pg/mL, IFNgamma: < 3 pg/mL, IL-1beta: < 5 pg/mL, IL-1RA: < 20 pg/mL, IL-4: < 1 pg/mL, IL-6: < 5 pg/mL, IL-10: < 2 pg/mL, IL-12p70: < 3 pg/mL, IL-17A: < 3 pg/mL, IL-18: < 5 pg/mL, IL-21: < 5 pg/mL, IL-22: < 3 pg/mL, MCP-1: < 2 pg/mL, TNFalpha: < 1 pg/mL.
Detection Method	Flow Cytometry
Assay Time	2 hours
1x Ab-conjugated beads (premixed)	S4P2 - anti-H IL-1beta, S4P4 - anti-H IL-6, S4P6 - anti-H TNFalpha, S4P7 - anti-H IL-22, S4P8 - anti-H IL-1RA, S4P9 - anti-H IFNgamma, S4P11 - anti-H MCP-1, S4P12 - anti-H IL-12p70, S5P3 - anti-H GM-CSF, S5P4 - anti-H IL-4, S5P6 - anti-H CXCL2, S5P7 - anti-H IL-17A, S5P9 - anti-H IL-18, S5P10 - anti-H IL-10, S5P11 - anti-H IFNalpha2, S5P12 - anti-H IL-21
2x Biotin-detection Ab (premixed)	Biotin - anti-H IL-1beta, Biotin - anti-H IL-6, Biotin - anti-H TNFalpha, Biotin - anti-H IL-22, Biotin - anti-H IL-1RA, Biotin - anti-H IFNgamma, Biotin - anti-H MCP-1, Biotin - anti-H IL-12p70, Biotin - anti-H GM-CSF, Biotin - anti-H IL-4, Biotin - anti-H CXCL2, Biotin - anti-H IL-17A, Biotin - anti-H IL-18, Biotin - anti-H IL-10, Biotin - anti-H IFNalpha2, Biotin - anti-H IL-21
Buffers	2x NR dAb Diluent. One vial containing 1.25 mL of biotin detection antibody diluent.
Lyophilized Standard Mix	One vial containing lyophilized recombinant CXCL2, GM-CSF, IFN?2, IFN?, IL-1?, IL-1RA, IL-4, IL-6, IL-10, IL-12p70, IL-17A, IL-18, IL-21, IL-22, MCP-1, and TNF?.
LLOQs	CXCL2: < 20 pg/mL, GM-CSF: < 10 pg/mL, IFNalpha2: < 10 pg/mL, IFN?: < 10 pg/mL, IL-1?: < 10 pg/mL, IL-1RA: < 40 pg/mL, IL-4: < 3 pg/mL, IL-6: < 10 pg/mL, IL-10: < 5 pg/mL, IL-12p70: < 10 pg/mL, IL-17A: < 10 pg/mL, IL-18: < 10 pg/mL, IL-21: < 10 pg/mL, IL-22: < 10 pg/mL, MCP-1: < 5 pg/mL, TNF?: < 2 pg/mL
ULOQs	CXCL2: > 5,000 pg/mL, GM-CSF: > 5,000 pg/mL, IFNalpha2: > 5,000 pg/mL, IFN ?: > 2,000 pg/mL, IL-1?: > 5,000 pg/mL, IL-1RA: > 15,000 pg/mL, IL-4: > 1,000 pg/mL, IL-6: > 5,000 pg/mL, IL-10: > 5,000 pg/mL, IL-12p70: > 2,000 pg/mL, IL-17A: > 1,000 pg/mL, IL-18: > 5,000 pg/mL, IL-21: > 5,000 pg/mL, IL-22: > 2,000 pg/mL, MCP-1: > 5,000 pg/mL, TNF?: > 1,000 pg/mL
Standard dose recovery	70-130%
Intra-assay CV	<10%
Inter-assay CV	<20%
Sample volume	15 µL/test

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