GeniePlex Human Cytokine 17-Plex Panel 1 (96 tests)

Catalog Number: AKME013

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Specifications

Product Information
Product Name	GeniePlex Human Cytokine 17-Plex Panel 1 (96 tests)
Analytes	GM-CSF IFNalpha2 IFNgamma IL-1beta IL-2 IL-4 IL-5 IL-6 IL-8 IL-9 IL-10 IL-12p70 IL-17A IL-22 IL-33 TNFalphaTSLP
Reactivity	Human
Sensitivity	GM-CSF: < 5 pg/mL, IFNalpha2: < 5 pg/mL, IFN?: < 3 pg/mL, IL-1?: < 5 pg/mL, IL-2: < 3 pg/mL, IL-4: < 1 pg/mL, IL-5: < 2 pg/mL, IL-6: < 5 pg/mL, IL-8: < 1 pg/mL, IL-9: < 5 pg/mL, IL-10: < 2 pg/mL, IL-12p70: < 3 pg/mL, IL-17A: < 3 pg/mL, IL-22: < 3 pg/mL, IL-33: < 5 pg/mL, TNF?: < 1 pg/mL, TSLP: < 2 pg/mL
Detection Method	Flow Cytometry
Assay Time	2 hours
1x Ab-conjugated beads (premixed)	S4P2 - anti-H IL-1beta, S4P3 - anti-H IL-10, S4P4 - anti-H TSLP, S4P6 - anti-H TNFalpha, S4P7 - anti-H IL-2, S4P9 - anti-H IFNgamma, S4P11 - anti-H IL-33, S4P12 - anti-H IL-12p70, S5P2 - anti-H IL-8, S5P3 - anti-H GM-CSF, S5P4 - anti-H IL-4, S5P5 - anti-H IL-5, S5P6 - anti-H IL-6, S5P7 - anti-H IL-17A, S5P8 - anti-H IL-22, S5P9 - anti-H IL-9, S5P11 - anti-H IFNalpha2
2x Biotin-detection Ab (premixed)	Biotin - anti-H IL-1beta, Biotin - anti-H IL-10, Biotin - anti-H TSLP, Biotin - anti-H TNFalpha, Biotin - anti-H IL-2, Biotin - anti-H IFNgamma, Biotin - anti-H IL-33, Biotin - anti-H IL-12p70, Biotin - anti-H IL-8, Biotin - anti-H GM-CSF, Biotin - anti-H IL-4, Biotin - anti-H IL-5, Biotin - anti-H IL-6, Biotin - anti-H IL-17A, Biotin - anti-H IL-22, Biotin - anti-H IL-9, Biotin - anti-H IFNalpha2
Buffers	2x NR dAb Diluent. One vial containing 1.25 mL of biotin detection antibody diluent.
Lyophilized Standard Mix	One vial containing lyophilized recombinant GM-CSF, IFNalpha2, IFNgamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-22, IL-33, TNFalpha and TSLP.
LLOQs	GM-CSF: < 10 pg/mL, IFNalpha2: < 10 pg/mL, IFN?: < 10 pg/mL, IL-1?: < 10 pg/mL, IL-2: < 10 pg/mL, IL-4: < 3 pg/mL, IL-5: < 5 pg/mL, IL-6: < 10 pg/mL, IL-8: < 1 pg/mL, IL-9: < 10 pg/mL, IL-10: < 5 pg/mL, IL-12p70: < 10 pg/mL, IL-17A: < 10 pg/mL, IL-22: < 10 pg/mL, IL-33: < 10 pg/mL, TNF?: < 2 pg/mL, TSLP: < 5 pg/mL
ULOQs	GM-CSF: > 5,000 pg/mL, IFNalpha2: > 5,000 pg/mL, IFN ?: > 2,000 pg/mL, IL-1?: > 5,000 pg/mL, IL-2: > 2,000 pg/mL, IL-4: > 1,000 pg/mL, IL-5: > 2,000 pg/mL, IL-6: > 5,000 pg/mL, IL-8: > 1,000 pg/mL, IL-9: > 5,000 pg/mL, IL-10: > 5,000 pg/mL, IL-12p70: > 2,000 pg/mL, IL-17A: > 1,000 pg/mL, IL-22: > 2,000 pg/mL, IL-33: > 5,000 pg/mL, TNF?: > 1,000 pg/mL, TSLP: > 2,000 pg/mL
Standard dose recovery	70-130%
Intra-assay CV	<10%
Inter-assay CV	<20%
Sample volume	15 µL/test

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