GeniePlex Human Cytokine 20-Plex Panel 1 (96 tests)

Catalog Number: AKME014

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Specifications

Product Information
Product Name	GeniePlex Human Cytokine 20-Plex Panel 1 (96 tests)
Analytes	6Ckine IL-1beta IL-2 IL-3 IL-4 IL-5 IL-6 IL-10 IL-11 IL-12p70 IL-15 IL-17A IL-18 IL-21 IL-25 IL-32 IL-33 IL-34 RANTESTECK
Reactivity	Human
Sensitivity	6Ckine: < 10 pg/mL, IL-1?: < 5 pg/mL, IL-2: < 3 pg/mL, IL-3: < 5 pg/mL, IL-4: < 1 pg/mL, IL-5: < 2 pg/mL, IL-6: < 5 pg/mL, IL-10: < 2 pg/mL, IL-11: < 5 pg/mL, IL-12p70: < 3 pg/mL, IL-15: < 5 pg/mL, IL-17A: < 3 pg/mL, IL-18: < 5 pg/mL, IL-21: < 5 pg/mL, IL-25: < 10 pg/mL, IL-32: < 10 pg/mL, IL-33: < 5 pg/mL, IL-34: < 5 pg/mL, RANTES: < 5 pg/mL, TECK: < 10 pg/mL
Detection Method	Flow Cytometry
Assay Time	2 hours
1x Ab-conjugated beads (premixed)	S4P2 - anti-H IL-1beta, S4P3 - anti-H IL-10, S4P4 - anti-H IL-6, S4P5 - anti-H IL-5, S4P6 - anti-H IL-2, S4P7 - anti-H IL-4, S4P8 - anti-H TECK, S4P9 - anti-H IL-15, S4P11 - anti-H IL-33, S4P12 - anti-H IL-12p70, S5P2 - anti-H IL-32, S5P3 - anti-H IL-25, S5P4 - anti-H IL-34, S5P5 - anti-H IL-17A, S5P6 - anti-H RANTES, S5P7 - anti-H IL-3, S5P8 - anti-H IL-18, S5P9 - anti-H IL-21, S5P10 - anti-H IL-11, S5P11 - anti-H 6Ckine
2x Biotin-detection Ab (premixed)	Biotin - anti-H IL-1beta, Biotin - anti-H IL-10, Biotin - anti-H IL-6, Biotin - anti-H IL-5, Biotin - anti-H IL-2, Biotin - anti-H IL-4, Biotin - anti-H TECK, Biotin - anti-H IL-15, Biotin - anti-H IL-33, Biotin - anti-H IL-12p70, Biotin - anti-H IL-32, Biotin - anti-H IL-25, Biotin - anti-H IL-34, Biotin - anti-H IL-17A, Biotin - anti-H RANTES, Biotin - anti-H IL-3, Biotin - anti-H IL-18, Biotin - anti-H IL-21, Biotin - anti-H IL-11, Biotin - anti-H 6Ckine
Buffers	2x NR dAb Diluent. One vial containing 1.25 mL of biotin detection antibody diluent.
Lyophilized Standard Mix	One vial containing lyophilized recombinant 6Ckine, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-11, IL-12p70, IL-15, IL-17A, IL-18, IL-21, IL-25, IL-32, IL-33, IL-34, RANTES and TECK.
LLOQs	6Ckine: < 20 pg/mL, IL-1?: < 10 pg/mL, IL-2: < 10 pg/mL, IL-3: < 10 pg/mL, IL-4: < 3 pg/mL, IL-5: < 5 pg/mL, IL-6: < 10 pg/mL, IL-10: < 5 pg/mL, IL-11: < 10 pg/mL, IL-12p70: < 10 pg/mL, IL-15: < 10 pg/mL, IL-17A: < 10 pg/mL, IL-18: < 10 pg/mL, IL-21: < 10 pg/mL, IL-25: < 20 pg/mL, IL-32: < 20 pg/mL, IL-33: < 10 pg/mL, L-34: < 10 pg/mL, RANTES: < 10 pg/mL, TECK: < 20 pg/mL
ULOQs	6Ckine: > 5,000 pg/mL, IL-1?: > 5,000 pg/mL, IL-2: > 2,000 pg/mL, IL-3: > 5,000 pg/mL, IL-4: > 1,000 pg/mL, IL-5: > 2,000 pg/mL, IL-6: > 5,000 pg/mL, IL-10: > 5,000 pg/mL, IL-11: > 5,000 pg/mL, IL-12p70: > 2,000 pg/mL, IL-15: > 5,000 pg/mL, IL-17A: > 1,000 pg/mL, IL-18: > 5,000 pg/mL, IL-21: > 5,000 pg/mL, IL-25: > 5,000 pg/mL, IL-32: > 5,000 pg/mL, IL-33: > 5,000 pg/mL, IL-34: > 5,000 pg/mL, RANTES: > 5,000 pg/mL, TECK: > 5,000 pg/mL
Standard dose recovery	70-130%
Intra-assay CV	<10%
Inter-assay CV	<20%
Sample volume	15 µL/test

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