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GeniePlex Human Th1/Th2/Th17 18-plex (96 Tests)

Catalog Number: AKME045

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Specifications

Product Information
Product Name GeniePlex Human Th1/Th2/Th17 18-plex (96 Tests)
Analytes GM-CSF IFN-Gamma IL-1-Beta IL-2 IL-4 IL-5 IL-6 IL-8 IL-9 IL-10 IL-12p70 IL-17A IL-17F IL-22 IL-33 TNF-Alpha TNF-Beta TSLP
Reactivity Human
Sensitivity GM-CSF: < 5 pg/mL,  IFN-Gamma: < 3 pg/mL,  IL-1-Beta: < 5 pg/mL,  IL-2: < 3 pg/mL,  IL-4: < 1 pg/mL,  IL-5: < 2 pg/mL,  IL-6: < 5 pg/mL,  IL-8: < 1 pg/mL,  IL-9: < 5 pg/mL,  IL-10: < 2 pg/mL,  IL-12p70: < 3 pg/mL,  IL-17A: < 3 pg/mL, IL-17F: < 2 pg/mL,  IL-22: < 3 pg/mL,  IL-33: < 5 pg/mL,  TNF-Alpha: < 1 pg/mL,  TNF-Beta: < 5 pg/mL,  TSLP: < 2 pg/mL
Detection Method Flow Cytometry
Assay Time 2 hours
1x Ab-conjugated beads (premixed) S4P2 - anti-human TNF-Beta, S4P3 - anti-human TNF-Alpha, S4P6 - anti-human IL-1-Beta, S4P7 - anti-human IL-2, S4P8 - anti-human IL-17F, S4P9 - anti-human IFN-Gamma, S4P10 - anti-human IL-8, S4P11 - anti-human IL-9, S4P12 - anti-human IL-12p70, S5P2 - anti-human IL-22, S5P3 - anti-human GM-CSF, S5P4 - anti-human IL-4, S5P5 - anti-human IL-5, S5P6 - anti-human IL-6, S5P7 - anti-human IL-33, S5P9 - anti-human IL-17A, S5P10 - anti-human IL-10, S5P11 - anti-human TSLP
2x Biotin-detection Ab (premixed) Biotin - anti-human TNF-Beta, Biotin - anti-human TNF-Alpha, Biotin - anti-human IL-1-Beta, Biotin - anti-human IL-2, Biotin - anti-human IL-17F, Biotin - anti -human IFN-Gamma, Biotin - anti-human IL-8, Biotin - anti-human IL-9, Biotin - anti-human IL-12p70, Biotin - anti-human IL-22, Biotin - anti-human GM-CSF, Biotin - anti-human IL-4, Biotin - anti-human IL-5, Biotin - anti-human IL-6, Biotin - anti-human IL-33, Biotin - anti-human IL-17A, Biotin - anti-human IL-10, Biotin - anti-human TSLP
Buffers 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix Lyophilized Standard Mix- Human TH1/Th2/Th17 18-plex. Vials A, B and C containing lyophilized recombinant human GM-CSF, IFN-Gamma, IL-1 Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, IL-33, TNF-Alpha, TNF Beta and TSLP.
LLOQs GM-CSF: <10 pg/mL, IFN-Gamma: <10 pg/mL, IL-1-Beta:  <10 pg/mL, IL-2: <10 pg/mL, IL-4: <3 pg/mL, IL-5: <5 pg/mL, IL-6: <10 pg/mL, IL-8: <1 pg/mL, IL-9: <10 pg/mL, IL-10: <5 pg/mL,  IL-12p70: <10 pg/mL, IL-17A: <10 pg/mL, IL-17F: <5 pg/mL, IL-22: <10 pg/mL, IL-33: <10 pg/mL, TNF-Alpha: <2 pg/mL, TNF-Beta: <10 pg/mL, TSLP: <5 pg/mL.
ULOQs GM-CSF:  > 5,000 pg/mL, IFN-Gamma: >2,000 pg/mL, IL-1-Beta: >5,000 pg/mL,  IL-2: >2,000 pg/mL, IL-4: >1,000 pg/mL, IL-5: >2,000 pg/mL, IL-6: >5,000 pg/mL, IL-8: >1,000 pg/mL, IL-9: >5,000 pg/mL, IL-10: >5,000 pg/mL, IL-12p70: >2,000 pg/mL,  IL-17A: >1,000 pg/mL, IL-17F: >2,000 pg/mL, IL-22: >2,000 pg/mL, IL-33: >5,000 pg/mL, TNF-Alpha: >1,000 pg/mL, TNF-Beta:  > 5,000 pg/mL, TSLP: >2,000 pg/mL.
Standard dose recovery 70-130%
Intra-assay CV Intra-assay CV: <10%
Inter-assay CV Inter-assay CV: <20%
Sample volume 15 µL/test

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