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GeniePlex Human Th1/Th2/Th17 7-Plex (96 Tests)

Catalog Number: AKME046

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Specifications

Product Information
Product Name GeniePlex Human Th1/Th2/Th17 7-Plex (96 Tests)
Analytes IFN-Gamma IL-2 IL-4 IL-6 IL-10 IL-17A TNF-Alpha
Reactivity Human
Sensitivity IFN-Gamma: < 3 pg/mL,  IL-2: < 3 pg/mL,  IL-4: < 1 pg/mL,  IL-6: < 5 pg/mL,  IL-10: < 2 pg/mL, IL-17A: < 3 pg/mL,  TNF-Alpha: < 1 pg/mL
Detection Method Flow Cytometry
Assay Time 2 hours
1x Ab-conjugated beads (premixed) S4P3 - anti-human TNF-Alpha, S4P7 - anti-human IL-2, S4P9 - anti-human IFN-Gamma, S5P4 - anti-human IL-4, S5P6 - anti-human IL-6, S5P9 - anti-human IL-17A, S5P10 - anti-human IL-10
2x Biotin-detection Ab (premixed) Biotin - anti-human TNF-Alpha, Biotin - anti-human IL-2, Biotin - anti -human IFN-Gamma, Biotin - anti-human IL-4, Biotin - anti-human IL-6, Biotin - anti-human IL-17A, Biotin - anti-human IL-10
Buffers 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix Lyophilized Standard Mix- Human Group 1 Panel A, 7-Plex. One vial containing lyophilized recombinant IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha.
LLOQs IFN-Gamma: <10 pg/mL, IL-2: <10 pg/mL, IL-4: <3 pg/mL, IL-6: <10 pg/mL, IL-10: <5 pg/mL,  IL-17A: <10 pg/mL, TNF-Alpha: <2 pg/mL.
ULOQs IFN-Gamma: >2,000 pg/mL, IL-2: >2,000 pg/mL, IL-4: >1,000 pg/mL, IL-6: >5,000 pg/mL, IL-10: >5,000 pg/mL, IL-17A: >1,000 pg/mL, TNF-Alpha: >1,000 pg/mL.
Standard dose recovery 70-130%
Intra-assay CV Intra-assay CV: <10%
Inter-assay CV Inter-assay CV: <20%
Sample volume 15 µL/test

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