GeniePlex Human Th1/Th2/Th17/Treg 10-Plex

Catalog Number: AKME048

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Specifications

Product Information
Product Name	GeniePlex Human Th1/Th2/Th17/Treg 10-Plex
Analytes	G-CSF GM-CSF IFN-Gamma IL-2 IL-4 IL-6 IL-10 IL-17A TNF-AlphaTNF-Beta
Reactivity	Human
Sensitivity	G-CSF: < 5 pg/mL, GM-CSF: < 5 pg/mL, IFN-Gamma: < 3 pg/mL, IL-2: < 3 pg/mL, IL-4: < 1 pg/mL, IL-6: < 5 pg/mL, IL-10: < 2 pg/mL, IL-17A: < 3 pg/mL, TNF-Alpha: < 1 pg/mL, TNF-Beta: < 5 pg/mL
Detection Method	Flow Cytometry
Assay Time	2 hours
1x Ab-conjugated beads (premixed)	S4P3 - anti-H IL-10, S4P4 - anti-H IL-6, S4P6 - anti-H TNFalpha, S4P7 - anti-H IL-2, S4P11 - anti-H TNFbeta, S5P2 - anti-H IFNgamma, S5P3 - anti-H GM-CSF, S5P4 - anti-H IL-4, S5P7 - anti-H IL-17A, S5P10 - anti-H G-CSF
2x Biotin-detection Ab (premixed)	Biotin - anti-H IL-10, Biotin - anti-H IL-6, Biotin - anti-H TNFalpha, Biotin - anti-H IL-2, Biotin - anti-H TNFbeta, Biotin - anti-H IFNgamma, Biotin - anti-H GM-CSF, Biotin - anti-H IL-4, Biotin - anti-H IL-17A, Biotin - anti-H G-CSF
Buffers	2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix	Lyophilized Standard Mix-Human TH1/Th2 10-Plex. One vial containing lyophilized recombinant G-CSF, GM-CSF, IFNgamma, IL-10, IL-17A, IL-2, IL-4, IL-6, TNFalpha and TNFbeta.
LLOQs	G-CSF: <10 pg/mL, GM-CSF: <10 pg/mL, IFN-Gamma: <10 pg/mL, IL-2: <10 pg/mL, IL-4: <3 pg/mL, IL-6: <10 pg/mL, IL-10: <5 pg/mL, IL-17A: <10 pg/mL, TNF-Alpha: <20pg/mL, TNF-Beta: <10 pg/mL.
ULOQs	G-CSF: >5,000 pg/mL, GM-CSF: >5,000 pg/mL, IFN-Gamma: >2,000 pg/mL, IL-2: >2,000 pg/mL, IL-4: >1,000 pg/mL, IL-6: >5,000 pg/mL, IL-10: >5,000 pg/mL, IL-17A: >1,000 pg/mL, TNF-Alpha: >1,000 pg/mL, TNF-Beta: >5,000 pg/mL..
Standard dose recovery	70-130%
Intra-assay CV	Intra-assay CV: <10%
Inter-assay CV	Inter-assay CV: <20%
Sample volume	15 µL/test

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