GeniePlex Mouse Inflammation 17-Plex (96 Tests)

Catalog Number: AKME052

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Specifications

Product Information
Product Name	GeniePlex Mouse Inflammation 17-Plex (96 Tests)
Analytes	IFN-Gamma IL-1-Alpha IL-1-Beta IL-6 IL-9 IL-10 IL-12p70 IL-13 IL-15 IL-23p19 IP-10 KC MCP-1 MIP-1-Alpha MIP-1-Beta RANTES TNF-Alpha
Reactivity	Mouse
Sensitivity	IFN-Gamma: <1 pg/mL, IL-1-Alpha: <2 pg/mL, IL-1-Beta: <2 pg/mL, IL-6: <3 pg/mL, IL-9: <5 pg/mL, IL-10: <2 pg/mL, IL-12p70: <1 pg/mL, IL-13: <5 pg/mL, IL-15: <5 pg/mL, IL-23p19: <5 pg/mL, KC: <1 pg/mL, IP-10: <3 pg/mL, MCP-1: <1 pg/mL, MIP-1-Alpha: <5 pg/mL, MIP-1-Beta: <5 pg/mL, RANTES : <2 pg/mL, TNF-Alpha: <0.5 pg/mL.
Detection Method	Flow Cytometry
Assay Time	2 hours
1x Ab-conjugated beads (premixed)	S4P2 - anti-mouse MIP-1-Beta; S4P3 - anti-mouse IL-23p19; S4P4 - anti-mouse TNF-Alpha; S4P6 - anti-mouse IL-1-Alpha; S4P7 - anti-mouse IL-12p70; S4P8 - anti-mouse IFN-Gamma; S4P9 - anti-mouse IL-9; S4P10 - anti-Mouse IP-10; S4P12 - anti-mouse IL-13; S5P2 - anti-mouse KC; S5P3 - anti-mouse MCP-1; S5P4 - anti-mouse IL-1-Beta; S5P5 - anti-mouse IL-6; S5P7 - anti-mouse MIP-1-Alpha; S5P8 - anti-mouse RANTES; S5P9 - anti-mouse IL-10; S5P12 - anti-mouse IL-15
2x Biotin-detection Ab (premixed)	Biotin - anti-mouse MIP-1-Beta; Biotin - anti-mouse IL-23p19; Biotin - anti-mouse TNF-Alpha; Biotin - anti-mouse IL-1-Alpha; Biotin - anti-mouse IL-12p70; Biotin - anti-mouse IFN-Gamma; Biotin - anti-mouse IL-9; Biotin - anti-Mouse IP-10; Biotin - anti-mouse IL-13; Biotin - anti-mouse KC; Biotin - anti-mouse MCP-1; Biotin - anti-mouse IL-1-Beta; Biotin - anti-mouse IL-6; Biotin - anti-mouse MIP-1-Alpha; Biotin - anti-mouse RANTES; Biotin - anti-mouse IL-10; Biotin - anti-mouse IL-15
Buffers	2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix	Lyophilized Standard Mix-Mouse Inflammation 17-Plex. One vial containing lyophilized recombinant IFN-Gamma, IL-1-Alpha, IL-1 Beta, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-23p19, IP-10, KC, MCP-1, MIP-1-Alpha, MIP-1 Beta, RANTES and TNF-Alpha.
LLOQs	IFN-Gamma, <2 pg/mL; IL-1-Alpha, <10 pg/mL; IL-1-Beta, <5 pg/mL; IL-6, < 10 pg/mL; IL-9, <10 pg/mL; IL-10, < 5 pg/mL; IL-12p70, <2 pg/mL; IL-13, <10 pg/mL; IL-15, <10 pg/mL; IL-23p19, <10 pg/mL; IP-10, <10 pg/mL; KC, <2 pg/mL; MCP-1, <2 pg/mL; MIP-1-Alpha, <10 pg/mL; MIP-1-Beta, <10 pg/mL; RANTES , <5 pg/mL; TNF-Alpha, <2 pg/mL. pg/mL
ULOQs	IFN-Gamma, >5,000 pg/mL; IL-1-Alpha, > 5,000 pg/mL; IL-1-Beta, >5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-9, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-12p70, >2,000 pg/mL; IL-13, >5,000 pg/mL; IL-15, >5,000 pg/mL; IL-23p19, >5,000 pg/mL; IP-10, >5,000 pg/mL; MCP-1, >2,000 pg/mL; KC, >2,000 pg/mL; MIP-1-Alpha, >5,000 pg/mL; MIP-1-Beta, >5,000 pg/mL; RANTES , <> 5,000 pg/mL; TNF-Alpha, >1,500 pg/mL..
Standard dose recovery	70-130%
Intra-assay CV	<10%
Inter-assay CV	<20%
Sample volume	15 µL/test

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