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GeniePlex Mouse Inflammation 8-Plex (96 Tests)

Catalog Number: AKME058

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Specifications

Product Information
Product Name GeniePlex Mouse Inflammation 8-Plex (96 Tests)
Analytes IFN-Gamma IL-1-Alpha IL-6 IL-10 IL-12p70 KC MCP-1 TNF-Alpha
Reactivity Mouse
Sensitivity IFN-Gamma: <1 pg/mL, IL-1-Alpha: <2 pg/mL, IL-6: <3 pg/mL, IL-10: <2 pg/mL, IL-12p70: <1 pg/mL,  KC: <1 pg/mL, MCP-1: <1 pg/mL, TNF-Alpha: <0.5 pg/mL.
Detection Method Flow Cytometry
Assay Time 2 hours
1x Ab-conjugated beads (premixed) S4P1 - anti-M IL-1-Alpha; S4P4 - anti-M TNF-Alpha; S4P7 - anti-M IL-12p70; S4P8 - anti-M IFN-Gamma; S5P2 - anti-M KC; S5P4 - anti-M MCP-1; S5P5 - anti-M IL-6; S5P9 - anti-M IL-10
2x Biotin-detection Ab (premixed) Biotin - anti-M IL-1-Alpha; Biotin - anti-M TNF-Alpha; Biotin - anti-M IL-12p70; Biotin - anti-M IFN-Gamma ; Biotin - anti-M KC; Biotin - anti-M MCP-1; Biotin - anti-M IL-6; Biotin - anti-M IL-10
Buffers 2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix Lyophilized Standard Mix-Mouse Inflammation 8-Plex. One vial containing lyophilized recombinant IFN-Gamma, IL-1-Alpha, IL-6, IL-10, IL-12p70, KC, MCP-1 and TNF-Alpha.
LLOQs IFN-Gamma, <2 pg/mL; IL-1-Alpha, <10 pg/mL; IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-12p70, <2 pg/mL;  KC, <2 pg/mL; MCP-1, <2 pg/mL; TNF-Alpha, <2 pg/mL. pg/mL
ULOQs IFN-Gamma, >5,000 pg/mL; IL-1-Alpha, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-12p70, >2,000 pg/mL;  MCP-1, >2,000 pg/mL; KC, >2,000 pg/mL; TNF-Alpha, >1,500 pg/mL..
Standard dose recovery 70-130%
Intra-assay CV <10%
Inter-assay CV <20%
Sample volume 15 µL/test

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