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Glucose Uptake Assay Kit - Fluorometric

Catalog Number: AKAS0310

Price: Online Inquiry

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Specifications

Product Information
Product Name Glucose Uptake Assay Kit - Fluorometric
Instruments Microplate Reader
Sample Type Cells
Research Area Glycolysis & Carbohydrates Glucose Uptake Diabetes & Obesity
Detection Principle 2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Assay Time 100 min
Storage -20°C
Shipping Conditions Gel Pack

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