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Glutaminase (GLS) Activity Assay Kit

Catalog Number: AKAS0318

Price: Online Inquiry

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Specifications

Product Information
Product Name Glutaminase (GLS) Activity Assay Kit
Detection Method Colorimetric method
Instruments Microplate Reader
Sample Type Serum, Plasma, Animal, Plant Tissue
Detection Principle Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.
Assay Time 80min
Other Reagents PBS (0.01 mol/L, pH 7.4)
Storage -20°C

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