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Glutamine Colorimetric Assay Kit

Catalog Number: AKAS0321

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Specifications

Product Information
Product Name Glutamine Colorimetric Assay Kit
Detection Method Colorimetric method
Instruments Microplate Reader
Sample Type Serum, Plasma, Tissue, Cells And Cell Culture Supernatan
Detection Principle Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.
Assay Time 70 min
Other Reagents Normal saline (0.9% NaCl)
Storage -20°C

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