Glutathione Peroxidase Activity Assay Kit

Catalog Number: AKAS0324

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Specifications

Product Information
Product Name	Glutathione Peroxidase Activity Assay Kit
Instruments	Microplate Reader
Sample Type	Serum, Plasma, Cells, Cell Culture Supernatant, Tissue
Research Area	Oxidative Stress Antioxidant Diabetes & Obesity Plant & Environmental Stress Enzyme Activity
Detection Principle	Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH- Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
Assay Time	40 min
Storage	2-8°C
Shipping Conditions	Gel Pack

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Please note that all services are for research use only. Not intended for any clinical use.

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