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Human Autophagy Array (20 targets)

Catalog Number: AKAA023

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Specifications

Product Information
Product Name Human Autophagy Array (20 targets)
Applications Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Analytes ATG12 ATG7 ATG10 ATG13 ATG3 ATG4A ATG4B ATG5 Beclin BNIP3L DDR2 GABARAP LAMP1 (CD107a) LC3a LC3B NBS1 P62 RHEB MSK1 Alpha-synuclein
ASSAY SIZE 2 Arrays/4 Arrays/8 Arrays/
Product Features Easy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technologyHighly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplex
Kit Components Human Autophagy Antibody Array C1 MembranesBlocking BufferWash Buffer 1Wash Buffer 2Biotinylated Detection Antibody CocktailStreptavidin-Conjugated HRPDetection Buffer CDetection Buffer DLysis Buffer8-Well Incubation TrayPlastic SheetsArray TemplatesOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline 1.Block membranes2.Incubate with Sample3.Incubate with Biotinylated Detection Antibody Cocktail4.Incubate with HRP-Conjugated Streptavidin5.Incubate with Detection Buffers6.Image with chemiluminescent imaging system7.Perform densitometry and analysis
Species Human
Number of Targets Detected 20
Gene Symbols ATG10, ATG12, ATG13, ATG3, ATG4A, ATG4B, ATG5, ATG7, BECN1, BNIP3L|DDR2, GABARAP, LAMP1, MAP1LC3A, MAP1LC3B, NBN, RHEB, RPS6KA5, SNCA, SQSTM1
Compatible Sample Types Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle Sandwich-based
Method of Detection Chemiluminescence
Quantitative/Semi-Quantitative Semi-Quantitative
Storage For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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