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Cas9 Expressing Human breast adenocarcinoma cells

Catalog Number: GEC0074

Price:
$3000
Specifications Overview Application Downloads Related products

Specifications

Product Information
Product Name Cas9 Expressing Human breast adenocarcinoma cells
Specification 1*10^6
System endocrine system
Resistance hygro
Cas9 expression detection Provided
Cell name SK-BR-3-CAS9
Cell morphology Epithelial like, adherent cells
passage ratio 1:2~1:3
Species expression gene Human
Expressed Gene Cas9
Properties
Construction method Viral method
Mycoplasma detection negative
Storage and transportation Dry ice transportation; Store in liquid nitrogen
Culture system 90% McCoy's 5A+10% FBS

Overview

SK-BR-3-CAS9 is a genetically engineered cell line derived from the SK-BR-3 breast cancer cell line modified with a CRISPR-Cas9 system to enable precise genome editing capabilities This product is particularly valuable for researchers investigating oncogenic mechanisms drug resistance and therapeutic interventions in breast cancer The CRISPR-Cas9 technology utilizes a guide RNA to direct the Cas9 nuclease to specific genomic loci allowing for targeted modifications such as gene knockout or insertion This facilitates the study of gene function and the identification of potential therapeutic targets with high specificity and efficiency

The scientific importance of SK-BR-3-CAS9 lies in its application in functional genomics and cancer research By providing researchers with a reliable tool for gene editing SK-BR-3-CAS9 can be employed to study the effects of various genetic alterations on tumorigenesis cell proliferation and response to treatment This capability makes it a crucial asset for developing novel therapeutics and understanding the molecular drivers of breast cancer

Compared to traditional methods of genome editing such as zinc finger nucleases or TALENs the SK-BR-3-CAS9 provides several advantages including ease of use higher precision and a reduced off-target effect Additionally its compatibility with high-throughput screening approaches allows for large-scale systematic studies increasing the throughput of research efforts

Researchers and clinicians alike will find SK-BR-3-CAS9 invaluable for its unique ability to elucidate complex interactions within cancer biology leading to groundbreaking discoveries and improved treatment modalities Our company specializes in the development and delivery of innovative biological products aimed at enhancing research efficacy and therapeutic development ensuring that you have access to the most advanced tools available

Application

SK-BR-3-CAS9 is primarily employed in cancer research and gene editing applications specifically in the study of breast cancer biology This product is particularly beneficial for researchers analyzing the mechanisms of HER2-positive breast cancer allowing for the targeted alteration of specific genes using CRISPR/Cas9 technology Researchers can implement SK-BR-3-CAS9 by transfecting it into SK-BR-3 cells where it facilitates the precise editing of genetic sequences enabling functional studies of oncogenes and tumor suppressor genes

In experimental contexts users have reported enhanced gene knockout efficiencies leading to the discovery of novel therapeutic targets and the validation of drug responses When integrated into workflows researchers benefit from the streamlined process of gene editing resulting in faster validation of hypotheses and more robust experimental results Specialized techniques such as high-throughput screening or the study of genetic interactions are notably optimized with SK-BR-3-CAS9 allowing for expanded capabilities in functional genomics studies and personalized medicine approaches

Downloads

Please note that all services are for research use only. Not intended for any clinical use.

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