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Cas9 Expressing Human breast cancer cells

Catalog Number: GEC0084

Price:
$1654.29
Specifications Overview Application Downloads Related products

Specifications

Product Information
Product Name Cas9 Expressing Human breast cancer cells
Specification 1*10^6
System endocrine system
Resistance hygro
Cas9 expression detection Provided
Cell name MCF7-CAS9
Cell morphology Epithelial like, adherent cells
passage ratio 1:2~1:4
Species expression gene Human
Expressed Gene Cas9
Properties
Construction method Viral method
Mycoplasma detection negative
Storage and transportation Dry ice transportation; Store in liquid nitrogen
Culture system 87% MEM+10% FBS+1% Glutamax+1% SodiumPyruvate+1% NEAA

Overview

MCF7-CAS9 is a genetically engineered cell line derived from the MCF7 human breast cancer cell line that incorporates the CRISPR-Cas9 gene-editing system This innovative tool enables researchers to precisely manipulate the genome of MCF7 cells facilitating the study of gene function protein interactions and the molecular mechanisms underlying breast cancer biology By utilizing the versatile CRISPR-Cas9 technology researchers can efficiently introduce targeted mutations deletions or insertions allowing for the exploration of specific genes' roles in cancer progression drug resistance and potential therapeutic targets

The MCF7-CAS9 cell line operates on the principles of homology-directed repair and non-homologous end joining enhancing the efficiency of genome editing Through the introduction of guide RNAs alongside the Cas9 nuclease this product enables specific targeting within the genome offering unparalleled control over gene modification This cell line serves as an invaluable platform in academic research and pharmaceutical development particularly in preclinical studies aimed at understanding tumor behavior and therapeutic responses

The scientific importance of MCF7-CAS9 is underscored by its applicability in various research fields including cancer biology gene therapy and regenerative medicine Its utility extends to drug discovery where the ability to create model systems with specific genetic alterations can lead to the identification of novel compounds or treatment strategies that are tailored to particular genetic backgrounds

Compared to traditional methods of RNA interference or other gene-editing technologies MCF7-CAS9 offers quicker more sustainable and robust gene-modulation capabilities Its flexibility and precision rival those of any comparable product making it a critical asset for researchers aiming to elucidate complex genetic interactions and their implications for cancer metabolism and therapy

For researchers and clinicians focusing on breast cancer MCF7-CAS9 represents an opportunity to advance their investigations with a cutting-edge tool that maximizes experimental precision while minimizing off-target effects The capability to develop tailored models and investigate versatile hypotheses enhances the overall understanding of breast cancer at a molecular level

At [Your Company Name] we pride ourselves on our extensive expertise in biological products continuously innovating to meet the evolving demands of researchers and clinicians alike MCF7-CAS9 epitomizes our commitment to advancing scientific discovery and improving clinical outcomes through high-quality reliable biological tools

Application

MCF7-CAS9 is a powerful biological tool primarily utilized in gene editing applications within cancer research and cell biology This derivative of the MCF7 breast cancer cell line is engineered with a CRISPR/Cas9 system enabling precise genome modifications to study gene function discover therapeutic targets and model cancer-related mutations Researchers employ MCF7-CAS9 to knock out or introduce specific genes facilitating investigations into tumorigenesis pathways and treatment resistance in breast cancer

In experimental workflows MCF7-CAS9 can be easily integrated into CRISPR-mediated protocols allowing for straightforward transfection with guide RNAs specific to genes of interest This streamlined application not only improves the efficiency of gene editing but also enhances reproducibility across experimental replicates The practical benefits of utilizing MCF7-CAS9 include accelerated insights into genetic interactions that influence breast cancer progression and enhanced validation of potential drug targets making it an essential component in advanced cancer research methodologies Additionally this cell line can be utilized in high-throughput screening assays further broadening its applications in pharmaceutical development and personalized medicine

Downloads

Please note that all services are for research use only. Not intended for any clinical use.

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