Human Cancer Discovery Array (30 targets)

Catalog Number: AKAA028

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Specifications

Product Information
Product Name	Human Cancer Discovery Array (30 targets)
Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Analytes	Alpha-fetoprotein Beta-2 Microglobulin bFGF CA125 CA19-9 CEA CRP (C-Reactive Protein) DKK-1 ErbB2 Ferritin FSH Galectin-3 GDF-15 HGF ICAM-1 (CD54) IL-8 (CXCL8) MIF MMP-2 NSE Osteoprotegerin (TNFRSF11B) Osteopontin (SPP1) PAI-1 PSA-free RANTES (CCL5) SCF TGF beta 1 Thrombospondin 1 TIMP-1 uPA VEGF-A
ASSAY SIZE	2 Arrays/4 Arrays/8 Arrays/
Product Features	Easy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technologyHighly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplex
Kit Components	Human Cancer Discovery Antibody Array C1 MembranesBlocking BufferWash Buffer 1Wash Buffer 2Biotinylated Detection Antibody CocktailStreptavidin-Conjugated HRPDetection Buffer CDetection Buffer DLysis Buffer8-Well Incubation TrayPlastic SheetsArray TemplatesOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	1.Block membranes2.Incubate with Sample3.Incubate with Biotinylated Detection Antibody Cocktail4.Incubate with HRP-Conjugated Streptavidin5.Incubate with Detection Buffers6.Image with chemiluminescent imaging system7.Perform densitometry and analysis
Species	Human
Number of Targets Detected	30
Gene Symbols	AFP, B2M, CA (19-9), CCL5, CEACAM5, CGA, CRP, CXCL8, DKK1, ENO2\|ERBB2, FGF2, FSHB, FTH1, GDF15, HGF, ICAM1, KITLG, KLK3, LGALS3, MIF, MMP2, MUC16, PRAP1, SERPINE1, SPP1, TGFB1, THBS1, TIMP1, TNFRSF11B, VEGFA
Compatible Sample Types	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Storage	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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