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Human CAR-T Cytokine Release Syndrome (CRS) 18-Plex Panel

Catalog Number: AKME078

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Specifications

Product Information
Product Name Human CAR-T Cytokine Release Syndrome (CRS) 18-Plex Panel
Analytes IL-2RA IL-1beta IL-6 IL-8 IL-10 MIP-1alpha MCP-1 IL-12p70 IFNgamma GM-CSF IL-4 IL-15 Reg3A Elafin ST-2 IL-17A TNFRI Granzyme B
Reactivity Human
Detection Method Flow Cytometry
Assay Time 2 hours
1x Ab-conjugated beads (premixed) S4P2 - anti-H IL-2RA, S4P3 - anti-H IL-1beta, S4P4 - anti-H IL-6, S4P6 - anti-H IL-8, S4P7 - anti-H IL-10, S4P9 - anti-H MIP-1alpha, S4P11 - anti-H MCP-1, S4P12 - anti-H IL-12p70, S5P2 - anti-H IFNgamma, S5P3 - anti-H GM-CSF, S5P4 - anti-H IL-4, S5P5 - anti-H IL-15, S5P6 - anti-H Reg3A, S5P7 - anti-H Elafin, S5P8 - anti-H ST-2, S5P9 - anti-H IL-17A, S5P10 - anti-H TNFRI, S5P12 - anti-H Granzyme B,
2x Biotin-detection Ab (premixed) Biotin - anti-H IL-2RA, Biotin - anti-H IL-1beta, Biotin - anti-H IL-6, Biotin - anti-H IL-8, Biotin - anti-H IL-10, Biotin - anti-H MIP-1alpha, Biotin - anti-H MCP-1, Biotin - anti-H IL-12p70, Biotin - anti-H IFNgamma, Biotin - anti-H GM-CSF, Biotin - anti-H IL-4, Biotin - anti-H IL-15, Biotin - anti-H Reg3A, Biotin - anti-H Elafin, Biotin - anti-H ST-2, Biotin - anti-H IL-17A, Biotin - anti-H TNFRI, Biotin - anti-H Granzyme B
Buffers 2x NR dAb Diluent. One vial containing 1.25 mL of biotin detection antibody diluent.
Lyophilized Standard Mix One vial containing lyophilized recombinant Elafin, GM-CSF, Granzyme B, IFNγ, IL-1β, IL-2RA, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-15, IL-17A, MCP-1, MIP-1α, Reg3A, ST-2, TNFRI.
LLOQs Elafin, < 50 pg/mL; GM-CSF, < 10 pg/mL; Granzyme B, < 20 pg/mL; IFNγ, < 10 pg/mL; IL-1β, < 10 pg/mL; IL-2RA, < 300 U/mL; IL-4, < 3 pg/mL; IL-6, < 10 pg/mL; IL-8, < 1 pg/mL; IL-10, < 5 pg/mL; IL-12p70, < 10 pg/mL; IL-15, < 10 pg/mL; IL-17A, < 10 pg/mL; MCP-1, < 5 pg/mL; MIP-1α, < 5 pg/mL; Reg3A, < 30 pg/mL; ST-2, < 5 pg/mL; TNFRI, < 10 pg/mL.
ULOQs Elafin, > 10,000 pg/mL; GM-CSF, > 5,000 pg/mL; Granzyme B, > 5,000 pg/mL; IFNγ, > 2,000 pg/mL; IL-1β, > 5,000 pg/mL; IL-2RA, > 150,000 U/mL; IL-4, > 1,000 pg/mL; IL-6, > 5,000 pg/mL; IL-8, > 1,000 pg/mL; IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-15, > 5,000 pg/mL; IL-17A, > 1,000 pg/mL; MCP-1, > 5,000 pg/mL; MIP-1α, > 2,000 pg/mL; Reg3A, > 10,000 pg/mL; ST-2, > 5,000 pg/mL; TNFRI, > 5,000 pg/mL.
Intra-assay CV < 10%
Inter-assay CV < 20%

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