Cas9 Expressing Human integrated SV40 gene in breast epithelial cells

Catalog Number: GEC0081

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$3428.57

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Specifications

Product Information
Product Name	Cas9 Expressing Human integrated SV40 gene in breast epithelial cells
Specification	1*10^6
System	endocrine system
Resistance	hygro
Cas9 expression detection	Provided
Cell name	HBL-100-CAS9
Cell morphology	Epithelial like, adherent cells
passage ratio	1:2~1:4
Species expression gene	Human
Expressed Gene	Cas9

Properties
Construction method	Viral method
Mycoplasma detection	negative
Storage and transportation	Dry ice transportation; Store in liquid nitrogen
Culture system	90% RPMI-1640+10% FBS

Overview

HBL-100-CAS9 is a cutting-edge genetic editing tool designed to facilitate precise modifications in the genomes of various organisms Leveraging the CRISPR-Cas9 technology HBL-100-CAS9 utilizes a guide RNA (gRNA) to direct the Cas9 nuclease to a specific DNA sequence where it creates double-strand breaks This targeted editing mechanism allows for either the disruption of genes or the introduction of new sequences through homology-directed repair enabling researchers to study gene function model diseases and develop novel therapies

The key functionality of HBL-100-CAS9 lies in its robustness and versatility across a range of biological systems By providing a high degree of specificity and efficiency researchers are able to manipulate genes with unprecedented precision This capability is vital in both basic research and clinical applications including gene therapy where the accurate correction of mutations can lead to groundbreaking treatments for genetic disorders Its applications extend to agriculture where precise genome editing can enhance crop resilience and yield

Compared to traditional gene editing methods HBL-100-CAS9 offers a more straightforward approach reducing off-target effects while increasing the speed of genetic modifications Its ease of delivery into cells and compatibility with a wide range of tissue types further distinguish it from other editing technologies Additionally researchers and clinicians can readily adopt this system due to its documented regulatory support and established protocols that simplify integration into existing workflows

The scientific importance of HBL-100-CAS9 does not just lie in its operational mechanism but also in its potential to catalyze new discoveries across genomics developmental biology and therapeutic research Its value to researchers and clinicians is significant providing a transformative platform to advance our understanding of genetics and inform tailored medical interventions

Our company boasts expertise in developing innovative biotechnological solutions and HBL-100-CAS9 exemplifies our commitment to advancing the field of genetic engineering By choosing HBL-100-CAS9 you are equipping your research or clinical practice with a powerful tool poised to make substantial contributions to science and medicine

Application

HBL-100-CAS9 is primarily applied in gene editing and functional genomics facilitating precise modifications of DNA sequences in research clinical and industrial settings This product excels in CRISPR/Cas9 applications enabling researchers to create knockouts knock-ins and specific point mutations in a variety of cell lines including human mouse and agricultural models In the laboratory researchers can easily incorporate HBL-100-CAS9 into their workflows by following established transfection protocols to introduce the Cas9 nuclease and guide RNA constructs into target cells

For instance when investigating gene function in cancer biology scientists can utilize HBL-100-CAS9 to validate therapeutic targets by generating specific gene knockouts leading to insight into oncogenic pathways The practical benefits of using HBL-100-CAS9 include streamlined gene editing processes high efficiency and reduced off-target effects significantly accelerating research timelines Moreover this product is compatible with various specialized techniques such as next-generation sequencing (NGS) for validating edit outcomes and single-cell analysis making it an invaluable asset in advanced genetic research

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Please note that all services are for research use only. Not intended for any clinical use.

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