Cas9 Expressing Human nasopharyngeal carcinoma cells

CNE1-CAS9 is an innovative and specialized cell line derived from human colorectal cancer (CRC) tissues meticulously engineered to constitutively express the Cas9 nuclease This product facilitates groundbreaking CRISPR/Cas9-based genome-editing applications specifically tailored for the study of colorectal cancer biology and therapeutic strategies The source cells are maintained in a standardized culture medium enriched with 10% fetal bovine serum (FBS) ensuring optimal growth under standard laboratory conditions

The stable integration of the Cas9 gene in CNE1-CAS9 is achieved through a lentiviral delivery system which promotes consistent expression and functional reliability The cell line exhibits robust performance in gene editing experiments providing researchers with a valuable tool for exploring genomic alterations associated with colorectal cancer Importantly the presence of hygromycin resistance allows for effective selection and expansion of Cas9-expressing cells facilitating experimental rigor and reproducibility

CNE1-CAS9 cells are crucial for a spectrum of applications including but not limited to gene function elucidation pathway characterization and validation of potential therapeutic targets pertinent to CRC The ability to employ CRISPR/Cas9 technology within this context contributes significantly to advancing personalized medicine approaches for colorectal cancer as researchers can dissect the functional roles of specific genes implicated in tumorigenesis and metastasis

To ensure experimental integrity CNE1-CAS9 cells undergo routine mycoplasma testing verifying their quality and suitability for sensitive genetic studies The cells are conveniently packaged and shipped in liquid nitrogen with transportation provided on dry ice to maintain viability and are delivered at a density of 1×10^6 cells per vial With optimal passage ratios of 1:3 to 1:5 recommended CNE1-CAS9 is designed for scalability enabling long-term studies and extensive screening processes in cancer research

In summary CNE1-CAS9 stands out as a significant tool for the scientific community empowering researchers to investigate the complexities of colorectal cancer through advanced genome-editing technologies Its unique composition and targeted applications not only enhance understanding of cancer biology but also hold promise for the development of novel therapeutic interventions