Product Information | |
---|---|
Product Name | Cas9 Expressing Human prostate cancer cells |
Specification | 1*10^6 |
System | endocrine system |
Resistance | hygro |
Cas9 expression detection | Provided |
Cell name | PC-3-CAS9 |
Cell morphology | Epithelial like, adherent cells |
passage ratio | 1:3~1:4 |
Species expression gene | Human |
Expressed Gene | Cas9 |
Properties | |
---|---|
Construction method | Viral method |
Mycoplasma detection | negative |
Storage and transportation | Dry ice transportation; Store in liquid nitrogen |
Culture system | 90% F12K+10% FBS |
PC-3-CAS9 is a state-of-the-art gene-editing tool based on the CRISPR-Cas9 technology platform specifically designed for precision genome modification in various biological systems This product harnesses the natural defense mechanisms of bacteria where CRISPR sequences and Cas9 proteins work in concert to target and cleave specific DNA sequences allowing for precise edits such as gene knockouts knock-ins and site-specific mutations PC-3-CAS9 employs engineered guide RNAs (gRNAs) that are customizable for targeting specific genes of interest thereby streamlining the gene editing process across diverse organisms
The technology behind PC-3-CAS9 operates through the formation of a ribonucleoprotein complex that binds to the target DNA sequence complementary to the gRNA Once bound the Cas9 endonuclease induces a double-strand break in the DNA facilitating the cell's natural repair mechanisms which can be exploited to integrate new genetic material or disrupt existing genes This dual mechanism highlights the therapeutic versatility and innovative research possibilities embodied by PC-3-CAS9
Scientifically PC-3-CAS9 holds significant value across both research and clinical settings from basic biological research to the development of gene therapies for genetic disorders Its applications extend to functional genomics biotechnology and synthetic biology providing researchers with powerful capabilities to elucidate gene functions and discover novel therapeutic targets
What distinguishes PC-3-CAS9 from alternative gene-editing tools such as TALENs or ZFNs is its unparalleled ease of use cost-effectiveness and high efficiency in generating targeted genetic modifications Additionally its modular design allows for rapid redesign of gRNAs enabling researchers to adapt it quickly to new research questions without extensive re-engineering efforts
Ultimately the value of PC-3-CAS9 for researchers and clinicians lies in its ability to facilitate groundbreaking advances in gene manipulation expand the boundaries of functional genomics and pave the way for innovative therapeutic strategies Our company combines years of expertise in molecular biology and biotechnology ensuring that PC-3-CAS9 remains at the forefront of gene-editing solutions for the scientific community
PC-3-CAS9 is primarily applied in gene editing and functional genomics serving both research laboratories and clinical settings This advanced CRISPR-Cas9 system is utilized for precise genome modification in various model organisms including human cell lines mouse models and agricultural species For instance researchers can employ PC-3-CAS9 to investigate gene function in disease models enabling the study of oncogenes in cancer research or genes involved in metabolic disorders
In a practical lab workflow scientists can implement PC-3-CAS9 by designing sgRNAs specific to their target genes and utilizing the product for transfection into cells This streamlined process allows for efficient knockout or knock-in studies yielding reproducible results that can accelerate discoveries
Benefits of using PC-3-CAS9 include enhanced editing accuracy reduced off-target effects and a significant reduction in time compared to traditional gene-editing methods Its efficacy in high-throughput screening applications and compatibility with standard laboratory protocols makes it an invaluable tool for advancing genetic research and therapeutic development
Please note that all services are for research use only. Not intended for any clinical use.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.