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Cas9 Expressing Human salivary gland adenoid cystic carcinoma cells

Catalog Number: GEC0002

Price:
$3428.57
Specifications Overview Application Downloads Related products

Specifications

Product Information
Product Name Cas9 Expressing Human salivary gland adenoid cystic carcinoma cells
Specification 1*10^6
System Eye, ear, nose, throat, and oral system
Resistance hygro
Cas9 expression detection Provided
Cell name SSACC-LM -CAS9
Cell morphology Epithelial like, adherent cells
passage ratio 1:3-1:5
Species expression gene Human
Expressed Gene Cas9
Properties
Construction method Viral method
Mycoplasma detection negative
Storage and transportation Dry ice transportation; Store in liquid nitrogen
Culture system 90% RPMI-1640+10% FBS

Overview

The SSACC-LM-CAS9 cell line maintains epithelial-like properties while adhering to surfaces and originates from human eye, ear, nose, throat, and oral tissues. The Cas9 protein continuously expresses through viral integration which leads to strong gene editing functionality. Hygromycin resistance in the cells allows researchers to create selective culture conditions. The cell line demonstrates active Cas9 expression while remaining free from mycoplasma contamination which provides a dependable and uncontaminated research model. Researchers move the cells using dry ice before placing them in liquid nitrogen to preserve them over time. Use RPMI-1640 at 90% concentration with 10% FBS and maintain a cell passage ratio between 1:3 and 1:5.

Application

Researchers can use the SSACC-LM-CAS9 cell line to conduct CRISPR-based gene editing research specifically on epithelial tissues from the eye, ear, nose, throat, and oral systems. Because this cell line maintains stable Cas9 expression researchers can achieve accurate and powerful gene editing to knock out, knock in or modify genes which proves essential for understanding gene function and disease pathology. This cell line serves as a research tool for understanding salivary gland cancers and oral cancers among other epithelial-derived diseases. The presence of a hygromycin resistance marker helps scientists identify transfected cells which improves the reliability of their experiments. The cell line serves as a strong platform for high-throughput screening applications while enabling functional genomics investigations and drug discovery research essential for analyzing therapeutic targets and molecular pathways.

Downloads

Please note that all services are for research use only. Not intended for any clinical use.

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