Cas9 Expressing Human tongue squamous cell carcinoma cells

The HSC3-CAS9 is a human oral squamous cell carcinoma (OSCC)-derived cell line designed for advanced genetic engineering applications This cell line has been genetically modified to express the Cas9 nuclease a hallmark of the CRISPR/Cas9 genome editing technology Originating from the HSC3 cell line known for its relevance in cancer research HSC3-CAS9 provides a robust platform for researchers aiming to dissect the genetic underpinnings of oral cancer biology and therapeutic resistance

Cultured in a nutrient-rich environment with RPMI-1640 medium complemented with 10% fetal bovine serum (FBS) HSC3-CAS9 exhibits optimal growth characteristics conducive to extensive experimentation The stable integration of the Cas9 gene is achieved using a lentiviral vector system ensuring a high level of enzymatic expression and facilitating effective genome modifications The presence of the Cas9 expression is confirmed through specific detection methods underscoring the reliability of this cell line for precise genetic manipulations

One of the standout features of HSC3-CAS9 is its hygromycin resistance which allows for the selective maintenance of Cas9-expressing populations This characteristic empowers researchers to easily enrich for genetically modified cells while minimizing contamination from non-transduced populations Rigorous mycoplasma testing guarantees the integrity and authenticity of the cell culture ensuring that experimental results are not confounded by microbial contamination

With its ability to serve as a model for OSCC studies HSC3-CAS9 is particularly valuable for the exploration of oncogenic pathways drug resistance mechanisms and novel therapeutic strategies Its application is also pivotal for functional genomics enabling researchers to create targeted gene knockouts or insertions to elucidate critical factors in the progression and treatment of oral cancers

HSC3-CAS9 cells are conveniently shipped and stored in liquid nitrogen with precautions for dry ice transport to maintain cell viability Each vial contains approximately 1×10^6 cells ensuring an ample supply for initial experiments For optimal results a recommended passage ratio of 1:2–1:4 is specified supporting consistent growth and functionality across multiple uses

This versatile cell line is an essential tool for researchers delving into the complexities of oral squamous cell carcinoma opening avenues for innovative therapies and advancing our understanding of cancer biology As the field continues to embrace genetic engineering technologies HSC3-CAS9 stands out as a pivotal resource in the quest for effective cancer treatments