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Hydroxyproline (HYP) Colorimetric Assay Kit

Catalog Number: AKAS0364

Price: Online Inquiry

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Specifications

Product Information
Product Name Hydroxyproline (HYP) Colorimetric Assay Kit
Detection Method Colorimetric method
Instruments Microplate Reader
Sample Type Serum, Animal Tissue, Urine
Detection Principle The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
Assay Time 50 min
Other Reagents 6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol
Storage 2-8°C

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