Biosynthesis In Silico and Experimental Validation

CD Biosynsis provides professional in silico and experimental validation services for synthetic biology researchers around the world to facilitate their cutting-edge research.

Introduction to in Silico and Experimental Validation

Once the sequences have been optimized for the target backbone, it is necessary to test whether these sequences can adopt the target structure. These sequences must be evaluated by computer through protein structure prediction methods before choosing to clear the computer-tested sequences for experimental verification. These sequences will follow the routine procedures for protein expression, purification and structural characterization using biophysical techniques (such as CD, fluorescence). More powerful tests include the structure determination using NMR, X-ray crystallography or even Cryo-EM.

Service Offering

Using our advanced equipment and top technology, CD Biosynsis is committed to providing high-quality one-stop in silico and experimental validation services from protein structural prediction, structural determination and analysis, to protein expression purification and identification to customers around the world.

Protein Structure Prediction

The prediction of protein structure from amino acid sequences is one of the most important and difficult tasks in computational biology and biophysics. With the help of computer simulation technology, we can provide professional protein structure prediction services using a series of prediction methods, including homology modeling, threading and ab initio modeling.

The workflow of protein structure prediction at CD Biosynsis.

Experimental Validation

• Gene Cloning

  — As a leader in providing molecular cloning service, CD Biosynsis can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic, or viral vectors.

• Protein Expression and Purification

  — With years of experience in recombinant protein expression, CD Biosynsis offers extensive expertise across a range of expression systems for producing your recombinant proteins, monoclonal antibodies, vaccine antigens or disease biomarkers.

• Structural Characterization

  — In terms of identifying the quality of protein products, protein structure characterization is the basis of biological development and continuous quality control. CD BioSicences provides structural characterization service to perform preliminary identification of de novo synthesized protein qualities.

• Structural Determination

  — CD Biosynsis offers dedicated services to determine the structure of proteins. Several methods are used to determine the protein structure, including X-ray crystallography, NMR and Cryo-EM.

The workflow of experimental validation of de novo protein design at CD Biosynsis.

Ordering Process

Welcome to contact us for project quotations and more detailed information.

For research use only. Not intended for any clinical use.

Reference

1. Knaff P M, et al. From In Silico to Experimental Validation: Tailoring Peptide Substrates for a Serine Protease. Biomacromolecules, 2020, 21(4): 1636-1643.

Please note that all services are for research use only. Not intended for any clinical use.