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α-Ketoglutarate Dehydrogenase(α-KGDH)Activity Assay Kit

Catalog Number: AKAS0615

Price: Online Inquiry

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Specifications

Product Information
Product Name α-Ketoglutarate Dehydrogenase(α-KGDH)Activity Assay Kit
Detection Method Colorimetric method
Instruments Microplate Reader
Sample Type Serum, Plasma, Animal And Plant Tissue
Detection Principle α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm
Assay Time 40 min
Storage -20°C

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Please note that all services are for research use only. Not intended for any clinical use.

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