Large Scale Protein Production Service

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We offer a comprehensive Large Scale Protein Production Service designed to meet the growing demands of pharmaceutical research, biotechnology, and industrial applications. Our service focuses on producing high-quality proteins in large quantities, leveraging state-of-the-art technology and extensive expertise in various expression systems. Whether you need proteins for drug development, biochemical assays, or industrial processes, our large scale production capabilities ensure you receive the quantities you need with the quality you require.

Cell line generation and development for cell culture processes forthe generation of recombinant proteins of interest(FM Wurm, et al.,2004)

Overview Service Process Examples and Solutions Frequently Asked Questions

Overview

Recombinant proteins are used throughout biological and biomedical science. Their production was once the domain of experts, but the development of simple, commercially available systems has made the technology more widespread. As a result, also more widespread is an appreciation of the difficult, strategic choices inherent to the process. Commonly confronted questions include: should the protein(s) be expressed in bacteria, in yeast, in insect cells or in human cells? Which expression vector should be used? If bacterial expression is used, which strain(s) should be chosen? Should one express the fulllength protein or a fragment thereof? Should the protein be tagged, and which affinity tag is the best? What is a good purification strategy, and what are the common pitfalls?

Large scale protein production involves the expression and purification of proteins in quantities sufficient for commercial or extensive research use. This process is essential for applications requiring large amounts of protein, such as drug manufacturing, therapeutic protein development, and large-scale biochemical studies. At CD Biosynsis, we utilize a variety of expression systems, including bacterial, yeast, insect, and mammalian cells, to ensure optimal production tailored to your specific needs.

Highlights:

Multiple Expression Systems: Flexibility to choose the best system for your protein.
Advanced Technology: State-of-the-art facilities and equipment for efficient production.
Comprehensive Support: From initial consultation to final delivery, we provide end-to-end support.

Comparison of Large Scale Protein Production Methods

Method	Description	Advantages	Limitations
Bacterial Expression	Protein production using bacterial hosts (e.g., E. coli).	High yield, cost-effective, fast growth.	Limited post-translational modifications.
Yeast Expression	Protein production using yeast systems (e.g., Pichia pastoris).	Capable of post-translational modifications, scalable.	Moderate yield, longer growth time.
Insect Cell Expression	Protein production using insect cell lines (e.g., Sf9).	High yield, complex post-translational modifications.	Higher cost, longer growth time.
Mammalian Cell Expression	Protein production using mammalian cells (e.g., CHO, HEK).	Best for complex proteins, human-like post-translational modifications.	Highest cost, slower growth time.

Service Process

Project Consultation: Collaborating with clients to understand specific protein production goals, including target protein, desired yield, and application requirements.
Expression System Selection: Recommending the most suitable expression system based on the protein's characteristics and project needs.
Gene Synthesis and Cloning: Synthesizing and cloning the gene of interest into an appropriate expression vector.
Optimization and Scale-Up: Optimizing expression conditions and scaling up production to achieve the desired yield.
Protein Purification: Utilizing advanced purification techniques to ensure high purity and functionality of the produced protein.
Quality Assurance: Conducting rigorous quality control assays to validate protein integrity and activity.
Delivery and Support: Delivering the purified protein with comprehensive documentation and providing ongoing technical support.

For more information on our Large Scale Protein Production Service, please fill out our online inquiry form. One of our specialists will get back to you promptly to discuss your specific needs.

Examples and Solutions

Case Examples	Solutions We Offer
Drug Development: Producing large quantities of therapeutic proteins for clinical trials.	High-yield production in mammalian cells to ensure human-like post-translational modifications.
Biochemical Assays: Generating bulk enzymes for large-scale biochemical studies.	Utilizing bacterial systems for cost-effective, high-yield enzyme production.
Industrial Applications: Producing proteins for use in industrial processes.	Tailoring production in yeast or bacterial systems to achieve scalability and cost-efficiency.
Vaccine Manufacturing: Producing antigens for vaccine development and production.	Optimizing yield and purity in insect or mammalian cell systems to meet regulatory standards.
Research and Development: Supplying research institutions with large quantities of specific proteins.	Providing flexible production options across different expression systems to meet diverse research needs.

Frequently Asked Questions

Q: What is large scale protein production?

A: Large scale protein production involves the expression and purification of proteins in quantities sufficient for commercial or extensive research use, ranging from grams to kilograms.

Q: What expression systems do you use for large scale protein production?

A: We use bacterial, yeast, insect, and mammalian cell systems to produce proteins, depending on the protein's characteristics and the project's requirements.

Q: How do you ensure the quality of proteins produced at large scale?

A: We conduct rigorous quality control assays, including purity, functionality, and stability tests, to ensure the highest quality of produced proteins.

Q: Can you produce proteins with post-translational modifications?

A: Yes, we can produce proteins with complex post-translational modifications using yeast, insect, and mammalian cell systems.

Q: What documentation do you provide with the produced proteins?

A: We provide detailed reports on production conditions, purification methods, and quality control results.

Q: What kind of support do you offer post-delivery?

A: We offer ongoing technical support and troubleshooting assistance to ensure the successful utilization of the produced proteins in your applications.

Please note that all services are for research use only. Not intended for any clinical use.

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